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Hatching of second-stage larvae of Toxocara canis: a rapid method for processing large numbers of worms

Published online by Cambridge University Press:  05 June 2009

N. S Crowcroft  and
S. H Gillespie
N. S Crowcroft
Affiliation:
University Division of Communicable Diseases, Royal Free Hospital School of Medicine, Rowland Hill St., London NW3 2QG, UK
S. H Gillespie*
Affiliation:
University Division of Communicable Diseases, Royal Free Hospital School of Medicine, Rowland Hill St., London NW3 2QG, UK
*
*Author to whom correspondence should be addressed.
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Abstract

A method for hatching second-stage larvae of Toxocara canis is described. Whole adult worms are processed by centrifugation in the presence of hypochlorite which allows the grinding step to be eliminated. The technique is more rapid and a high yield is obtained.

Keywords

Toxocara canishatching technique
Type
Research Note
Information
Journal of Helminthology , Volume 65 , Issue 4 , December 1991 , pp. 311 - 312
Copyright
Copyright © Cambridge University Press 1991

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References

REFERENCES

De Savigny, D. H. (1975) In vitro maintenance of Toxocara canis larvae and a simple method for the production of Toxocara ES antigen for use in serodiagnostic tests for visceral larva migrans. Journal of Parasitology, 61, 781–782.CrossRefGoogle Scholar
De Savigny, D. H. & Tizzard, J. R. (1977) Toxocara larva migrans: the use of larval secretory antigens in haemagglutination and soluble antigen fluorescent antibody tests. Transactions of the Royal Society of Tropical Medicine and Hygiene, 71, 501–507.CrossRefGoogle ScholarPubMed
Smith, H. V. (1989) A rapid method for hatching infective eggs of Toxocara canis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 83, 215.CrossRefGoogle ScholarPubMed