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Genetic characterization of Echinococcus granulosus strains isolated from humans based on nad1 and cox1 gene analysis in Isfahan, central Iran

Published online by Cambridge University Press:  06 November 2017

R. Jafari ,
B. Sanei ,
A. Baradaran ,
A. Spotin ,
B. Bagherpour  and
H. Yousofi Darani
R. Jafari
Affiliation:
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
B. Sanei
Affiliation:
Department of Surgery, Isfahan University of Medical Sciences, Isfahan, Iran
A. Baradaran
Affiliation:
Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
A. Spotin
Affiliation:
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
B. Bagherpour
Affiliation:
Acquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
H. Yousofi Darani*
Affiliation:
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
*
Author for correspondence: H. Yousofi Darani, E-mail: yousofidarani@gmail.com
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Abstract

Cystic echinococcosis (CE) is a medically important parasite-caused human disease. Humans may acquire the infection accidentally by ingestion of E. granulosus eggs. The parasite has a broad range of hosts and genotypes, which may affect its aetiological and biological characteristics. The present study aimed to determine the genetic characteristics of human isolates of E. granulosus in Isfahan, Iran. In this cross-sectional study, 50 surgically removed hydatid cysts were collected from hospitalized patients in Al-Zahra Hospital, Isfahan, Iran, over a period of 2 years (2015–2017). DNA was extracted from cyst material, and polymerase chain reactions (PCR) were performed targeting cox1 and nad1 genes. Amplicons were sequenced directly and the resulting sequences were aligned and analysed. Phylogenetic and genetic diversity analyses were also performed. Among the isolates, 43 (86%), 3 (6%) and 4 (8%) out of 50 were E. granulosus (G1), E. granulosus (G3) and E. intermedius (G6), respectively. In total, nine and eight haplotypes were identified by nad1 and cox1 gene analysis, respectively. The haplotype diversity index was higher by cox1 gene analysis (0.547) in G1 strains compared with nad1 (0.433). The G1 genotype was the most predominant isolate from human cases of CE, and the presence of G6 is indicative of an important role of camels in the development of human CE in Isfahan. This is the first report of the G3 genotype causing human CE in Isfahan. Moreover, cox1 gene analysis enables a higher resolution of the genetic diversity of the E. granulosus population compared with nad1 gene analysis.

Type
Research Paper
Information
Journal of Helminthology , Volume 92 , Issue 6 , November 2018 , pp. 696 - 702
Copyright
Copyright © Cambridge University Press 2017 

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