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Frequency of Toxocara canis antibodies in Mexican paediatric patients with epilepsy

Published online by Cambridge University Press:  23 September 2019

M.d.L. Caballero-García ,
J. Simón-Salvador ,
J.C. Hernández-Aguilar ,
A. Reyes-Lopez ,
B. Nogueda-Torres  and
E. Jiménez-Cardoso Open the ORCID record for E. Jiménez-Cardoso [Opens in a new window]
M.d.L. Caballero-García
Affiliation:
Laboratorio de Investigación en Parasitología, Hospital Infantil de México Federico Gómez, Dr. Márquez, 162, 06720 Mexico, Mexico
J. Simón-Salvador
Affiliation:
Laboratorio de Investigación en Parasitología, Hospital Infantil de México Federico Gómez, Dr. Márquez, 162, 06720 Mexico, Mexico
J.C. Hernández-Aguilar
Affiliation:
Departamento de Neurología, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, 06720 Mexico, Mexico
A. Reyes-Lopez
Affiliation:
Medicina Basada en Evidencias, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, 06720 Mexico, Mexico
B. Nogueda-Torres
Affiliation:
Departamento de Parasitología, Escuela Nacional de Ciencias Biológica, Instituto Politécnico Nacional
E. Jiménez-Cardoso*
Affiliation:
Laboratorio de Investigación en Parasitología, Hospital Infantil de México Federico Gómez, Dr. Márquez, 162, 06720 Mexico, Mexico
*
Author for correspondence: E. Jiménez-Cardoso, E-mail: enedina@unam.mx
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Abstract

The relationship between epilepsy and the presence of visceral larva migrans caused by Toxocara canis in Mexican children remains uncertain; however, this relationship needs to be elucidated because these parasite larvae can invade the human central nervous system. Accordingly, this study aimed to determine the frequency and specificity of anti-T. canis antibodies in the sera of children with epilepsy to determine the relationship between this parasite and epilepsy. The sera samples of 214 children were examined: 111 children diagnosed with epilepsy and 103 clinically healthy children without neurological disorders. In the sera of each group, the presence and specificity of anti-T. canis and anti-Ascaris lumbricoides antibodies, as well as the cross-reactivity between them, were assessed using enzyme-linked immunosorbent assay and Western blotting analysis. Among the children with epilepsy, 25.2% exhibited seropositivity to T. canis. Cross-reactivity against the A. lumbricoides antigen was present in 46.8% of the children with epilepsy, whereas 11.7% of the children with epilepsy and anti-T. canis antibodies did not exhibit cross-reactivity against this antigen. The Western blotting analysis of the sera from the children with epilepsy demonstrated the presence of T. canis proteins, with molecular weights of 24, 35, 55, 70, 120 and 210 kDa, and A lumbricoides proteins with molecular weights of 70, 80 and 110 kDa. Our results revealed the presence of anti-T. canis antibodies in the children with epilepsy; furthermore, cross-reactivity tests with A. lumbricoides showed the importance of the presence of anti-T. canis antibodies in revealing the relationship between this parasite and epilepsy in children.

Keywords

Toxocara canisepilepsyserologyantigensantibodies
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e89
Copyright
Copyright © Cambridge University Press 2019 

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References

Ávila, AJM (2004) Comorbilidad del trastorno compulsivo en pacientes con epilepsia. Archivos de Neurociencias (Mex., DF) 9, 94–99.Google Scholar
Bächli, H, Minet, JC and Gratzi, O (2004) Cerebral toxocariasis: as possible cause of epileptic seizure in children. Child's Nervous System 63, 301–387.Google Scholar
Badley, JE, Grieve, RB, Bowman, DD, Glickman, LT and Rockey, JH (1987) Analysis of Toxocara canis larval excretory-secretory antigens: physicochemical characterization and body recognition. The Journal of Parasitology 73, 593–600.Google Scholar
Beaver, PC (1969) The nature of visceral larva migrans. The Journal of Parasitology 55, 3–12.Google Scholar
Bellanger, AP, Humbert, P, Gavignet, B, Deschaseaux, AD, Barisien, C, Roussel, S, Millon, L, Aubin, F and Piarroux, R (2010) Comparative assessment of enzyme-linked immunosorbent assay and Western blot for the diagnosis of toxocariasis in patients with skin disorders. The British Journal of Dermatology 162, 80–82.Google Scholar
Bradford, MM (1976) A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry 72, 248–254.Google Scholar
Cardona, NI, Fabian, L and Gomez, JE (2005) Estandarización del inmunoensayo ELISA para la detección de IgG anti-Toxoplasma gondii en ratón. Parasitología latinoamericana 60, 97–101.Google Scholar
Commission on Classification and Terminology of the International League against Epilepsy (1993) Guidelines for epidemiologic studies on epilepsy. Epilepsia 34, 592–596.Google Scholar
Critchley, EM, Vakil, SD, Hutchinson, DN and Taylor, P (1982) Toxoplasma, Toxocara and epilepsy. Epilepsia 23, 315–323.Google Scholar
Despommier, D (2003) Toxocariasis: clinical aspects, epidemiology, medical ecology, and molecular aspects. Clinical Microbiology Reviews 16, 265–272.Google Scholar
Encalada-Mena, LA, Duarte-Ubaldo, EI, Vargas-Magaña, JJ, García-Ramírez, MJ and Medina-Hernández, RE (2011) Prevalencia de parásitos gastroentéricos de cánidos en la ciudad de Escarcega, Campeche, México. Universidad y Ciencia 27, 209–217.Google Scholar
Eraky, MA, Abdel-Hady, S and Abdallah, KF (2016) Seropositivity of Toxoplasma gondii and Toxocara spp. in children with cryptogenic epilepsy, Benha, Egypt. The Korean Journal of Parasitology 54, 335–338.Google Scholar
Espinoza, Y, Huapaya, P, Suárez, R, Chávez, V, Sevilla, C, Dávila, E, Huiza, A, Náquira, C and Alva, P (2003) Estandarización de la técnica de ELISA para el diagnóstico de la toxocariasis humana. Anales de la Facultad de Medicina 64, 7–12.Google Scholar
Fitz, BMYF, da Silva, RR, Pelegi, ZB, de Lima, CLM, Pereira, L and Alvares, SV (2018) Dispersion and infectivity of Toxocara canis eggs after passage through chicken intestine. Parasitology Research 117, 3481–3486.Google Scholar
Glickman, LT, Cypess, RH, Crumrine, PK and Gitlin, DA (1979) Toxocara infection and epilepsy in children. The Journal of Pediatrics 94, 75–78.Google Scholar
Iddawela, RD, Rajapakse, RPVJ, Perera, NA and Agatsuma, T (2007) Characterization of a Toxocara canis species-specific excretory-secretory antigen (TcES-57) and development of a double sandwich ELISA for diagnosis of visceral larva migrans. The Korean Journal of Parasitology 45, 19–26.Google Scholar
Kennedy, MW, Qureshi, F, Fraser, EM, Haswell-Elkins, MR, Elkins, DB and Smith, HV (1989) Antigenic relationships between the surface-exposed, secreted and somatic materials of the nematode parasites Ascaris lumbricoides, Ascaris suum, and Toxocara canis. Clinical and Experimental Immunology 75, 493–500.Google Scholar
Laemmli, UK and Favre, M (1973) Maturation of the head of bacteriophage T4 I. DNA packaging events. Journal of Molecular Biology 80, 575–599.Google Scholar
Magnaval, JF, Fabre, R, Mauriéres, P, Charlet, JP and Larrard, B (1991) Application of the Western blotting procedure for the immunodiagnosis of human toxocariasis. Parasitology Research 77, 697–702.Google Scholar
Martínez-Barbabosa, I, Fernandez-Presas, AM, Vazquez-Tsuji, O and Ruíz-Hernández, A (1998) Frecuencia de Toxocara canis en perros y áreas verdes del sur de la ciudad de México, Distrito Federal. Veterinaria México 29, 239–244.Google Scholar
Nicoletti, A, Bartoloni, A, Sofia, V, Mantella, A, Nsengiyumva, G, Frescaline, G and Preux, PM (2007) Epilepsy ant toxocariasis: a case-control study in Burundi. Epilepsia 48, 894–899.Google Scholar
Nicoletti, A, Bartoloni, A, Regio, A, et al. (2002) Epilepsy, cisticercosis and toxocariasis: a population based case-control study in rural Bolivia. Neurology 58, 1256–1261.Google Scholar
Nicoletti, A, Sofia, V, Mantella, A, et al. (2008) Epilepsy and toxocariasis: a case-control study in Italy. Epilepsia 49, 594–599.Google Scholar
Oaks, AJ and Kayes, SG (1979) Artificial hatching and culture of Toxocara canis second stage larvae. The Journal of Parasitology 65, 969–970.Google Scholar
Qualizza, R, Incorvaia, C, Grande, R, Makri, E and Allegra, L (2011) Seroprevalence of IgG anti-Toxocara species antibodies in a population of patients with suspected allergy. International Journal of General Medicine 4, 783–787.Google Scholar
Robertson, BD, Bianco, AT, McKerrow, JH and Maizels, RM (1989) Toxocara canis: proteolytic enzymes secreted by the infective larva in vitro. Experimental Parasitology 6, 30–36.Google Scholar
Roldán, HW and Espinoza, AY (2009) Evaluation of an enzyme-linked immunoelectrotransfer blot test for the confirmatory serodiagnosis of human toxocariasis. Memorias do Instituto Oswaldo Cruz 104, 411–418.Google Scholar
Romero, NC, García, CA, Mendoza, MGD, Torres, CNC and Ramírez, DN (2009) Contaminación por Toxocara spp, en parques de Tulyehualco, México. Revista Científica 19, 1–5.Google Scholar
Romero, NC, Mendoza, MGD, Bustamante, LP, Crosby, GMM and Ramírez, DN (2011) Presencia y viabilidad de Toxocara spp en suelos de parques públicos, jardines de casas y heces de perros en Nezahualcoyolt, México. Revista Científica 21, 195–201.Google Scholar
Romero, NC, Mendoza, MGD, Yañez, AS, Ponce, MM and Bustamante, MP (2013a) Prevalence and risk factors associated with Toxocara canis infection in children. The Scientific World Journal 2011, 1–5.Google Scholar
Romero, NC, Yañez, AS, Mendoza, MGD, Bustamante-Montes, LP and Ramírez-Durán, N (2013b) Contaminación y viabilidad de huevos de Toxocara spp. En suelo y heces colectadas en parque públicos, calles y perros en Toluca, México. Revista Científica 6, 475–479.Google Scholar
Rosamanta, A, Romero, JL and Arias, M (2003) Diagnosis of parasitic zoonoses by immunoenzymatic assays analysis of cross-reactivity among the excretory/secretory antigens of Fasciola hepatica, Toxocara canis and Ascaris suum. Immunology Investigations 32, 131–142.Google Scholar
Santillán, G, Bastin, V, Céspedes, G and Monkiewicz, A (2013) Evaluación de un antígeno purificado para el diagnóstico de toxocariosis. Revista Argentina de Microbiología 45, 80–82.Google Scholar
Savigny, DH (1975) In vitro maintenance of Toxocara canis larvae and a simple method for the production of Toxocara ES antigen for use in serodiagnostic tests for visceral larva migrans. The Journal of Parasitology 61, 781–782.Google Scholar
Speiser, F and Gottstein, BA (1984) Collaborative study on larval secretory-excretory antigens of Toxocara canis for the immunodiagnosis of human toxocariasis with ELISA. Acta Tropica 41, 361–372.Google Scholar
Towbin, H, Staehelin, T and Gordon, J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings National Academy of Sciences USA 76, 4350–4354.Google Scholar
Viovy, AA, Jofré, ML, Noemi, HI, Erazo, TR and Cerva, CJL (1999) Granuloma cerebral por Toxocara: Comunicación de caso clínico. Revista Chilena de Infectología 15, 312–316.Google Scholar