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Fate of developing larvae of Onchocerca lienalis and O.volvulus in micropore chambers implanted into laboratory hosts

Published online by Cambridge University Press:  05 June 2009

A. E. Bianco ,
M. B. Mustafa  and
P. J. Ham
A. E. Bianco
Affiliation:
Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts AL4 0XQ, UK
M. B. Mustafa
Affiliation:
Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts AL4 0XQ, UK
P. J. Ham
Affiliation:
Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts AL4 0XQ, UK
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Abstract

Infective larvae of Onchocerca lienalis and O.volvulus implanted subcutaneously within micropore chambers into laboratory hosts moulted to the fourth stage (L4) and underwent limited development and growth. Similar recoveries of O. lienalis L4 larvae in the range of 33–66% were obtained from chambers implanted into CBA and BALB/c strains of mice, jirds, and the natural bovine host. A relatively constant proportion of larvae survived up to 24 days post implantation and thereafter recoveries declined, although some worms were still alive after 96 days. Recoveries of O. volvulus L4 larvae from chambers given to normal or T-cell deprived mice were equivalent to one another and to those obtained with O. lienalis. Moulting of O. lienalis in chambers was observed on days 3 and 5, in close accordance with the timing of the third moult in cattle following systemic infection. Moulting of O. volvulus occurred between days 3–6. Morphological changes in developing larvae included a small but significant increase in length, a transient increase in width, and early development of the spicular primordia and genital tube. L4 larvae of O. lienalis, but not those of O. volvulus, exhibited 3 distinct caudal papillae not present on infective larvae.

Keywords

Onchocerca lienalisOnchocerca volvulusmouseMeriones unguiculatussubcutaneous implantationdevelopment
Type
Research Article
Information
Journal of Helminthology , Volume 63 , Issue 3 , September 1989 , pp. 218 - 226
Copyright
Copyright © Cambridge University Press 1989

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