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Expression of Hsp90, Hsp70 and Hsp60 in Trichinella species exposed to oxidative shock

Published online by Cambridge University Press:  12 April 2024

J. Martinez ,
J. Perez-Serrano ,
W.E. Bernadina  and
F. Rodriguez-Caabeiro
J. Martinez*
Affiliation:
Facultad de Farmacia, Departamento de Microbiologia y Parasitologia, Universidad de Alcala, 28871 Alcala de Henares, Madrid, Spain
J. Perez-Serrano
Affiliation:
Facultad de Farmacia, Departamento de Microbiologia y Parasitologia, Universidad de Alcala, 28871 Alcala de Henares, Madrid, Spain
W.E. Bernadina
Affiliation:
Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht, PO Box 80165, 3508 TD Utrecht, The Netherlands
F. Rodriguez-Caabeiro
Affiliation:
Facultad de Farmacia, Departamento de Microbiologia y Parasitologia, Universidad de Alcala, 28871 Alcala de Henares, Madrid, Spain
*
*Fax: 34 91 8854663 E-mail: francisco.martinez@uah.es.
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Abstract

Stress response and phosphorylation of heat shock proteins (HSPs) 60, 70 and 90 were studied in Trichinella nativa, T. nelsoni, T. pseudospiralis and T. spiralis larvae at 30-min intervals following exposure to 20, 100 and 200 mM H2O2. There was a time- and dose-dependent differential survival for the infective stage larvae (L1) of these four Trichinella species. Immunoblotting analysis revealed that constitutive Hsp60 and Hsp70, but not Hsp90, from test Trichinella species are constitutively phosphorylated on serine/threonine residues as they converted to forms with increased sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) mobility by treatment with alkaline phosphatase. After exposure to H2O2, while there was a time-related occurrence of the three HSPs with decreased SDS–PAGE mobility, these HSPs were insensitive to alkaline phosphatase except in the case of exposure to 20 mM H2O2 for Hsp60 from all Trichinella species and Hsp70 from T. spiralis and T. nelsoni. The synthesis of HSPs forms with decreased SDS–PAGE mobility is a susceptibility signal because the lower concentration of peroxide (20 mM) did not cause a decrease on HSPs SDS–PAGE mobility in T. spiralis and T. nelsoni, the two more resistant selected Trichinella species.

Type
Other
Information
Journal of Helminthology , Volume 76 , Issue 3 , September 2002 , pp. 217 - 223
Copyright
Copyright © Cambridge University Press 2002

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