Hostname: page-component-848d4c4894-v5vhk Total loading time: 0 Render date: 2024-06-21T03:07:03.741Z Has data issue: false hasContentIssue false
  • English
  • Français

Experimental transfer of adult Oesophagostomum dentatum from donor to helminth naive recipient pigs: a methodological study

Published online by Cambridge University Press:  05 June 2009

H. Bjørn ,
A. Roepstorff ,
C. Grøndahl ,
L. Eriksen ,
J. Bjerregaard  and
P. Nansen
H. Bjørn
Affiliation:
Danish Centre for Experimental Parasitology, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
A. Roepstorff
Affiliation:
Danish Centre for Experimental Parasitology, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
C. Grøndahl
Affiliation:
Department of Clinical Studies, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
L. Eriksen
Affiliation:
Department of Clinical Studies, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
J. Bjerregaard
Affiliation:
The Pharmacy, Department of Pharmacology and Pathobiology, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
P. Nansen
Affiliation:
Danish Centre for Experimental Parasitology, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
Get access Rights & Permissions [Opens in a new window]

Abstract

This study was carried out to compare potential methods of transplanting adult Oesophagostomum dentatum from experimentally infected donor pigs to helminth naive recipient pigs. The following methods were each tested in five pigs: A. Transfer of worms by stomach tube to the gastric ventricle of pigs per os pretreated with 0.5 mg/kg cisapride to increase gastrointestinal peristalsis; B. Transfer by stomach tube to the gastric ventricle of pigs per os pre-treated with cisapride (0.5 mg/kg) and omeprazol 20 mg which blocks hydrochloric acid secretion; C. Surgical transfer of worms to caecum of pigs. Worms for transplantation to pigs were obtained after slaughter of experimentally infected donor pigs and following isolation from the contents of the large intestine, using an agar gel migration technique. A mean of 1054 nematodes were transferred into each recipient pig within 2 hours. Procedures A and B resulted in establishment rates corresponding to only 0.5% and 7.6% of the transferred worms. In contrast, surgical transfer allowed 74.2% of the transplanted worms to be established. In all groups the transplanted worms migrated to the normal predilection site, i.e. the middle part` of the large intestine. More female than male worms established in all groups. It was concluded from this study that surgical transfer was the most reliable of the methods tested for experimental establishment of adult O. dentatum in helminth naive pigs.

Type
Research Papers
Information
Journal of Helminthology , Volume 69 , Issue 4 , December 1995 , pp. 279 - 283
Copyright
Copyright © Cambridge University Press 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bjørn, H., Roepstorff, A., Waller, P.J., & Nansen, P. (1990) Resistance to levamisole and cross-resistance between pyrantel and levamisole in Oesophagostomum quadrispinulatum and Oesophagostomum dentatum of pigs. Veterinary Parasitology 37, 21–30.CrossRefGoogle ScholarPubMed
Bråten, T. & Hopkins, C.A. (1969) The migration of Hymenolepis diminuta in the rat's intestine during normal development and following surgical transplantation. Parasitology 59, 891–905.CrossRefGoogle ScholarPubMed
Christensen, N.Ø., Knudsen, J. & Andreassen, J. (1986) Echinostoma revolutum: resistance to secondary and superimposed infections in mice. Experimental Parasitology, 61, 311–318.CrossRefGoogle ScholarPubMed
Jørgensen, R.J. (1975) In vitro migration of Dictyocaulus viviparus larvae: migration of the infective stage in agargel. International Journal for Parasitology, 5, 199–202.CrossRefGoogle Scholar
Le Jambre, L.F., Royal, W.M. & Martin, P.J. (1979) The inheritance of thiabendazole resistance in Haemonchus contortus. Parasitology 78, 107–121.CrossRefGoogle ScholarPubMed
Le Jambre, L.F. (1979) Hybridization studies of Haemonchus contortus (Rudolphi, 1803) and H. placei (Place, 1893), (Nematoda: Trichostrongylidae). International Journal for Parasitology 9, 455–463.CrossRefGoogle Scholar
Lejambre, L.F. (1981) Hybridization ofAustralianHaemonchus placei (Place, 1893), Haemonchus contortus cayugensis (Das and Whitlock, 1960) and Haemonchus contortus (Rudolphi,1803) from Lousiana. International Journal for Parasitology 11, 323–330.CrossRefGoogle Scholar
McKellar, Q., Duncan, J.L. & Armour, J. (1986) Response to transplanted adult Ostertagia ostertagi in calves. Research in Veterinary Science 40, 367–371.CrossRefGoogle ScholarPubMed
McKellar, Q., Duncan, J.L., Armoui, J. & Lindsay, ELF. (1987)Further studies on the response to transplanted adult Ostertagia ostertagi in calves. Research in Veterinary Science 40, 367–371.CrossRefGoogle Scholar
Ogilvie, B.M. (1965) Role of adult worms in immunity of rats to Nippostrongylus brasiliensis. Parasitology 55, 325–335.CrossRefGoogle ScholarPubMed
Roepstorff, A., Bjørn, H. & Nansen, p. (1987) Resistance of Oesophagostomum spp. in pigs to pyrantel citrate. Veterinary Parasitology 24, 229–239.CrossRefGoogle ScholarPubMed
Suarez, V.H., Cabaret, J. & Gasnier, N. (1992) A method for studying interbreeding of nematodes of the subfamily Ostertagiinae in sheep with abomasal fistulae. Veterinary Parasitology, 41 93–99.CrossRefGoogle ScholarPubMed
Scott, E. W., McKellar, Q.A., Armour, J., Bairden, K. & Bogan, J. A. (1988) Production of strains of the sheep parasite Ostertagia circumcincta by implantation of adult parasites into the abomasum of lambs. Research in Veterinary Science 45, 120–121.CrossRefGoogle ScholarPubMed
van Wyk, J. A. & Gerber, H.M. (1978) Recovery of nematodes from ruminants by migration from gastro-intestinal ingesta and mucosa gelled in agar: preliminary report. Onderstepoort Journal of Veterinary Research, 45, 29–38.Google ScholarPubMed
van Wyk, J.A., Gerber, H.M. & Groeneveld, H.T. (1980) A technique for the recovery of nematodes from ruminants by migration from gastro-intestinal ingesta gelled in agar: large scale application. Onderstepoort Journal of Veterinary Research 47, 147–158.Google ScholarPubMed