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Experimental infection routes of Angiostrongylus cantonensis in mice

Published online by Cambridge University Press:  05 June 2009

Lian-Chen Wang ,
David Chao  and
Eng-Rin Chen
Lian-Chen Wang
Affiliation:
Department of Biology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan, Republic of China Department of Parasitology, Kaohsiung Medical College, Kaohsiung 80424, Taiwan, Republic of China
David Chao*
Affiliation:
Department of Biology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan, Republic of China
Eng-Rin Chen
Affiliation:
Department of Parasitology, Kaohsiung Medical College, Kaohsiung 80424, Taiwan, Republic of China
*
‡Author for correspondence.
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Abstract

Stomach intubation is the most common method used in the experimental infection of animals with Angiostrongylus cantonensis. In order to compare the effectiveness of other possible transmission methods, groups of BALB/c mice were given infective third-stage larvae of A. cantonensis by different routes including intraperitoneal or subcutaneous injections, and penetration of anal mucosa, vaginal mucosa, conjunctival mucosa, lacerated skin, unabraded skin, foot pad and tail skin, while stomach intubation was used as control. Recovery of fifth-stage larvae was higher in mice inoculated with third-stage larvae subcutaneously. Successful infections were established through all experimental transmission routes except tail skin penetration. This study suggests that oral infection may not be the only route for the transmission of human angiostrongyliasis, and subcutaneous infection may be a better method for experimental infection.

Keywords

Angiostrongylus cantonensismouseinfection routeexperimental infection
Type
Research Papers
Information
Journal of Helminthology , Volume 65 , Issue 4 , December 1991 , pp. 296 - 300
Copyright
Copyright © Cambridge University Press 1991

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