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Evaluation of antioxidant and oxidant status of goats (Capra aegagrus hircus) naturally infected with Haemonchus contortus

Published online by Cambridge University Press:  14 February 2019

S. Rashid Open the ORCID record for S. Rashid [Opens in a new window]  and
M. Irshadullah Open the ORCID record for M. Irshadullah [Opens in a new window]
S. Rashid*
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh-202002, India
M. Irshadullah
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh-202002, India
*
Author for correspondence: S. Rashid E-mail: sadiarashid.27@gmail.com
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Abstract

The present study aimed to assess the antioxidant and oxidant status of goats naturally infected with Haemonchus contortus. Based upon the parasite burden, infection in goats was categorized as heavy (> 500 worms), mild (100–500 worms) or low (< 100 worms). Abomasal tissues from non-infected and infected goats were used for the determination of catalase (CAT), glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx), aspartate (AST) and alanine (ALT) aminotransferases, acid (ACP) and alkaline (ALP) phosphatases, superoxide content (O2−), protein carbonyl (PC), malondialdehyde (MDA) and reduced glutathione (GSH). A significantly higher level of CAT, GST and GR activity and a lower level of GPx activity were recorded in infected compared to non-infected tissue. A significant increase in the level of AST, ALT, ALP and ACP was found in the abomasal tissue of the infected animals, which was related to the worm burden. The oxidative stress markers were also altered, with a significant decline in GSH levels, whereas MDA, PC and O2− concentrations showed a marked increase. In conclusion, it has been demonstrated that haemonchosis in goats resulted in considerable oxidative stress, which was directly related to the worm burden.

Keywords

Antioxidant enzymesgoatsHaemonchus contortusoxidative stress
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e36
Copyright
Copyright © Cambridge University Press 2019 

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