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Effect of praziquantel on the strobilar development of Mesocestoides corti in vitro

Published online by Cambridge University Press:  12 April 2024

L. Britos ,
L. Domínguez ,
R. Ehrlich  and
M. Marín
L. Britos
Affiliation:
Facultad de Ciencias, Sección Bioquímica, Iguá 4225, Montevideo, 11400, Uruguay
L. Domínguez
Affiliation:
Facultad de Química, Departamento de Farmacología, Gral Flores 2124, Montevideo, Uruguay
R. Ehrlich
Affiliation:
Facultad de Ciencias, Sección Bioquímica, Iguá 4225, Montevideo, 11400, Uruguay
M. Marín*
Affiliation:
Facultad de Ciencias, Sección Bioquímica, Iguá 4225, Montevideo, 11400, Uruguay
*
*Author for correspondence Fax: +598 2 525 86 17 E-mail: marin@fcien.edu.uy
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Abstract

The effect of praziquantel (PZQ) on the strobilar development of the cyclophyllidean cestode Mesocestoides corti was explored. Mesocestoides corti larvae were cultivated under conditions reported to favour their differentiation to the adult stage. Parasites were exposed to 0.1 μg ml-1 PZQ for 16 h and subsequently transferred to drug-free medium. The ocurrence of segmentation — an early event of the larval somatic differentiation to the adult worm — was considered as quantitative data. This phenomenon was evidenced earlier in worms transiently exposed to PZQ with respect to control cultures. Moreover, the rate of segmentation of drug-treated worms at the end of the experiment almost doubled that of control worms. To date, no similar effect on any cestode developmental process has been reported for an anthelmintic drug. In the light of the existing knowledge and understanding of PZQ mechanisms of action, the proposed experimental approach could contribute to the elucidation of pathways and mechanisms involved in cestode strobilar development.

Type
Research Article
Information
Journal of Helminthology , Volume 74 , Issue 4 , December 2000 , pp. 295 - 299
Copyright
Copyright © Cambridge University Press 2000

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