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Effect of mutagen on cultured Schistosoma mansoni

Published online by Cambridge University Press:  05 June 2009

G. C. Coles  and
J. Fitzgerald
G. C. Coles
Affiliation:
Department of Zoology, University of Massachusetts, Amherst, MA 01003-0027, USA
J. Fitzgerald
Affiliation:
Department of Zoology, University of Massachusetts, Amherst, MA 01003-0027, USA
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Abstract

Although lightly homogenized three-week-old Schistosoma mansoni incubated in Mistuhashi and Maramorosch insect tissue culture medium or the medium of Weller and Wheeldon produced adherent cell layers, continued growth of these cells did not occur. Non-adherent cells obtained by trypsinization also failed to produce long term cell cultures even after the addition of a range of growth factors. The possibility of producing tumour-like schistosome cells by the use of the mutagen ethyl methane sulphonate was therefore examined. Four-hour exposure of three-week-old schistosomes caused in some worms (a) large fluid filled ‘ballooning’, which also occurred in adult males, (b) enlargement of the gut, (c) increase in numbers of large round cells within the worms and (d) tissus outgrowths. It is suggested that these effects of mutagen offer new approaches to obtaining permanent schistosome cell cultures.

Keywords

Schistosoma mansonimutagenscell culture
Type
Research Papers
Information
Journal of Helminthology , Volume 60 , Issue 2 , June 1986 , pp. 135 - 142
Copyright
Copyright © Cambridge University Press 1986

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