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Effect of agitation speed on the density of bacteria Photorhabdus luminescens and the population dynamics of nematodes Heterorhabditis megidis in liquid culture

Published online by Cambridge University Press:  10 September 2021

D. Tumialis ,
A. Mazurkiewicz Open the ORCID record for A. Mazurkiewicz [Opens in a new window]  and
I. Skrzecz
D. Tumialis
Affiliation:
Department of Animal Environment Biology, Institute of Animal Sciences, Warsaw University of Life Sciences – SGGW, Ciszewskiego 8, 02-786Warsaw, Poland
A. Mazurkiewicz*
Affiliation:
Department of Animal Environment Biology, Institute of Animal Sciences, Warsaw University of Life Sciences – SGGW, Ciszewskiego 8, 02-786Warsaw, Poland
I. Skrzecz
Affiliation:
Department of Forest Protection, Forest Research Institute, Braci Leśnej 3 Street, Sękocin Stary, 05-090Raszyn, Poland
*
Author for correspondence: A. Mazurkiewicz, E-mail: anna_mazurkiewicz@sggw.edu.pl
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Abstract

Liquid culture is the most scalable technology for the industrial production of entomopathogenic nematodes. Variability of the recovery after inoculation into cultures of Photorhabdus luminescens remains a persistent problem in the mass production of Heterorhabditis sp. In order to enhance infective juvenile (IJ) recovery and improve nematode population management, we analysed the correlation between the nematode Heterorhabditis megidis (strain KV – 136) development in liquid cultures, the density of bacteria of P. luminescens and the culture agitation speed. Analyses focused on the impact of different agitation speeds (160 rpm and 200 rpm) on the dynamics of population growth of H. megidis in liquid cultures at constant biotic and abiotic parameters (initial dose of nematodes introduced to the culture 2300 IJs/ml, temperature 25°C, the number of bacterial colonies 0.3 × 107/ml). The performed experiments showed that the agitation speed of 200 rpm favourably affected the density of bacteria of P. luminescens (24.14 × 107/ml). High density of bacteria at this agitation speed resulted in an earlier (on the fifth day of the culture) maximum increase in the number of hermaphroditic individuals (1239.6 H/ml) than in the culture at an agitation speed of 160 rpm.

Keywords

Entomopathogenic nematodesHeterorhabditis megidisPhotorhabdus luminescenspopulation dynamicsliquid culture
Type
Research Paper
Information
Journal of Helminthology , Volume 95 , 2021 , e54
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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