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Dirofilaria immitis: the moulting of the infective larva in vitro

Published online by Cambridge University Press:  18 November 2009

E. Devaney
E. Devaney
Affiliation:
Department of Parasitology, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK.
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Abstract

A number of in vitro culture systems were tested for their ability to support the development of Dirofilaria immitis infective larvae to the fourth larval stage. In cultures of medium ML-15 containing a feeder layer of Dog Sarcoma (DS) cells larvae successfully moulted and showed a small but significant increase in length. Ultrastructural observations demonstrated that the fourth-stage cuticle was synthesized in vitro and in some larvae was fully formed by 60 hours of culture. The hypodermis of moulting larvae contained numerous multi-vesicular bodies. It is concluded that the moult in vitro is a true moult and not an atypical response of the larvae to the conditions of culture.

Type
Research Papers
Information
Journal of Helminthology , Volume 59 , Issue 1 , March 1985 , pp. 47 - 50
Copyright
Copyright © Cambridge University Press 1985

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