Hostname: page-component-77c89778f8-sh8wx Total loading time: 0 Render date: 2024-07-17T13:51:05.376Z Has data issue: false hasContentIssue false
  • English
  • Français

Development and evaluation of a colloidal gold immunochromatographic assay based on recombinant protein CatL1D for serodiagnosis of sheep fasciolosis

Published online by Cambridge University Press:  04 November 2019

W. Xifeng ,
Q. Mengfan ,
Z. Kai ,
Z. Guowu ,
L. Jing ,
W. Lixia ,
Q. Jun ,
M. Qingling ,
G. Shasha  and
H. Yunfu
...Show all authors
W. Xifeng
Affiliation:
College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China
Q. Mengfan
Affiliation:
School of Biotechnology, Central South University, Changsha, Hunan 410012, China
Z. Kai
Affiliation:
College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China
Z. Guowu
Affiliation:
College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China
L. Jing
Affiliation:
College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China
W. Lixia
Affiliation:
College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China
Q. Jun
Affiliation:
College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China
M. Qingling*
Affiliation:
College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China
G. Shasha
Affiliation:
College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China
H. Yunfu
Affiliation:
College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China
C. Xuepeng
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China
*
Author for correspondence: M. Qingling, E-mail: xjmqlqj@sina.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Fasciolosis is a zoonotic parasitic disease that seriously endangers the development of animal husbandry and human health. In order to develop a rapid serological diagnostic method for fasciolosis in ruminants, the CatL1D and CatB4 genes of Fasciola hepatica were amplified by reverse transcription polymerase chain reaction (PCR) and cloned, respectively, and then the CatL-B fusion gene (MeCatL-B) was constructed by gene splicing by overlap extension PCR technique. The recombinant rCatL1D, rCatB4 and rMeCatL-B proteins were then prepared by prokaryotic expression, respectively, and the recombinant protein with high specificity and sensitivity was screened via indirect enzyme-linked immunosorbent assay. Using the selected recombinant protein rCatL1D as a diagnostic antigen, we developed a colloidal gold immunochromatographic assay (CGIA) for detecting F. hepatica-specific antibodies, and 426 serum samples of slaughtered sheep were used to evaluate the sensitivity and specificity of F. hepatica CGIA assay. The results showed that the sensitivity and specificity of rCatL1D protein (100%, 96.67%) were higher than those of rCatB4 (94.29%, 80%) and rMeCatL-B (91.43%, 90%). Compared with the gold standard post-mortem inspection, the specificity and sensitivity of the CGIA method was 100% and 97%, respectively, and the consistency rate between these two methods was 99.3%. These results confirmed that the CGIA method based on rCatL1D protein could be a promising approach for rapid diagnosis of sheep fasciolosis because of its high sensitivity and specificity.

Keywords

Fasciola hepaticarCatL1DrCatB4rMeCatL-Bindirect ELISAreactogenicitycolloidal gold immunochromatography assay (CGIA)
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e98
Copyright
Copyright © Cambridge University Press 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

These authors contributed equally

References

Abdolahi Khabisi, S, Sarkari, B, Moshfe, A, et al. (2017) Production of monoclonal antibody against excretory-secretory antigen of Fasciola hepatica and evaluation of is efficacy in the diagnosis of fascioliasis. Monoclonal A ntibodies in Immunodiagnosis and Immunotherapy 36, 8–14.Google Scholar
Aguayo, V, Valdes, B, Espino, AM, et al. (2018) Assessment of Fasciola hepatica glutathione S-transferase as an antigen for serodiagnosis of human chronic fascioliasis. Acta Tropica 186, 41–49.Google Scholar
Carnevale, S, Rodríguez, MI, Santillán, G, et al. (2001) Immunodiagnosis of human fascioliasis by an enzyme-linked immunosorbent assay (ELISA) and a micro-ELISA. Clinical and Diagnostic Laboratory Immunology 8, 174–177.Google Scholar
Castro, E, Freyre, A and Hernández, Z (2000) Serological responses of cattle after treatment and during natural re-infection with Fasciola hepatica, as measured with a dot-ELISA system. Veterinary Parasitology 90, 201–208.Google Scholar
Chung, YB, Kong, Y, Joo, IJ, et al. (1995) Excystment of Paragonimus westermani metacercariae by endo genous cysteine protease. Journal of Parasitology 81, 137–142.Google Scholar
Collins, PR, Stack, CM, O'Neill, SM, et al. (2004) Cathepsin L1, the major protease involved in liver fluke (Fasciola hepatica) virulence: propetide cleavage sites and autoactivation of the zymogen secreted from gastrodermal cells. Journal of Biological Chemistry 279, 17038–17046.Google Scholar
Cornelissen, JB, de Leeuw, WA and van der Heijden, PJ (1992) Comparison of an indirect haemagglutination assay and an ELISA for diagnosing Fasciola hepatica in experimentally and naturally infected sheep. Veterinary Quarterly 14, 152–156.Google Scholar
Fox, NJ, White, PC, McClean, CJ, et al. (2011) Predicting impacts of climate change on Fasciola hepatica risk. PLoS One 6, e16126.Google Scholar
George, SD, Vanhoff, K, Baker, K, et al. (2017) Application of a coproantigen ELISA as an indicator of efficacy against multiple life stages of Fasciola hepatica infections in sheep. Veterinary Parasitology 246, 60–69.Google Scholar
Jayaraj, R, Piedrafita, D, Dynon, K, et al. (2009) Vaccination against fasciolosis by a multivalent vaccine of stage-specific antigens. Veterinary Parasitology 160, 230–236.Google Scholar
Kelley, JM, Elliott, TP, Beddoe, T, et al. (2016) Current threat of triclabendazole resistance in Fasciola hepatica. Trends in Parasitology 32, 458–469.Google Scholar
Kong, MM, Yang, B, Gong, CJ, et al. (2017) Development of immunochromatographic colloidal gold test strip for rapid detection of Haemophilus influenzae in clinical specimens. Journal of Applied Microbiology 123, 287–294.Google Scholar
Marcos, LA, Yi, P, Machicado, A, et al. (2007) Hepatic fibrosis and Fasciola hepatica infection in cattle. Journal of Helminthology 81, 381–386.Google Scholar
Mas-Coma, S (2005) Epidemiology of fascioliasis in human endemic areas. Journal of Helminthology 79, 207–216.Google Scholar
Mas-Coma, S, Valero, MA and Bargues, MD (2009) Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control. Advances in Parasitology 69, 41–146.Google Scholar
McGonigle, L, Mousley, A, Marks, NJ, et al. (2008) The silencing of cysteine proteases in Fasciola hepatica newly excysted juveniles using RNA interference reduces gut penetration. International Journal for Parasitology 38, 149–155.Google Scholar
Meemon, K and Sobhon, P (2015) Juvenile-specific cathepsin proteases in Fasciola spp.: their characteristics and vaccine efficacies. Parasitology Research 114, 2807–2813.Google Scholar
Mehmood, K, Zhang, H, Sabir, AJ, et al. (2017) A review on epidemiology, global prevalence and economical losses of fasciolosis in ruminants. Microbial Pathogenesis 109, 253–262.Google Scholar
Mirzadeh, A, Valadkhani, Z, Yoosefy, A, et al. (2017) Expression, purification and in vitro refolding of the recombinant truncated saposin like protein 2 antigen for development of diagnosis of human fascioliasis. Acta Tropica 71, 163–171.Google Scholar
Mokhtarian, K, Meamar, AR, Khoshmirsafa, M, et al. (2018) Comparative assessment of recombinant and native immunogenic forms of Fasciola hepatica proteins for serodiagnosis of sheep fasciolosis. Parasitology Research 117, 225–232.Google Scholar
Molina-Hernández, V, Mulcahy, G, Pérez, J, et al. (2015) Fasciola hepatica vaccine: we may not be there yet but we're on the right road. Veterinary Parasitology 208, 101–111.Google Scholar
Moreau, E, Hervé, S, Yu, ZW, et al. (2002) Modulation of sheep lymphocyte responses by Fasciola hepatica excretory-secretory products. Veterinary Parasitology 108, 207–215.Google Scholar
Piedrafita, D, Parsons, JC, Sandeman, RM, et al. (2001) Antibody-dependent cell-mediated cytotoxicity to newly excysted juvenile Fasciola hepatica in vitro is mediated by reactive nitrogen intermediates. Parasite Immunology 23, 473–482.Google Scholar
Robinson, MW, Menon, R, Donnelly, SM, et al. (2009) An integrated transcriptomics and proteomics analysis of the secretome of the helminth pathogen Fasciola hepatica: proteins associated with invasion and infection of the mammalian host. Molecular & Cellular Proteomics 8, 1891–1907.Google Scholar
Rondelaud, D, Dreyfuss, G and Vignoles, P (2006) Clinical and biological abnormalities in patients after fasciolosis treatment. Medecine et Maladies Infectieuses 36, 466–468.Google Scholar
Salimi-Bejestani, MR, McGarry, JW, Felstead, S, et al. (2005) Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test. Research in Veterinary Science 78, 177–181.Google Scholar
Sampaio-Silva, ML, Da Costa, JM, Da Costa, AM, et al. (1996) Antigenic components of excretory-secretory products of adult Fasciola hepatica recognized in human infections. American Journal of Tropical Medicine and Hygiene 54, 146–148.Google Scholar
Shin, SH, Hsu, A, Chastain, HM, et al. (2016) Development of two Fh SAP2 recombinant-based assays for immunodiagnosis of human chronic fascioliasis. American Journal of Tropical Medicine and Hygiene 95, 852–855.Google Scholar
Shrifi, Y, Farahnak, A, Golestani, A, et al. (2014) Triclabendazole effect on protease enzyme activity in the excretory-secretory products of Fasciola hepatica in vitro. Iranian Journal of Parasitology 9, 107–113.Google Scholar
Solana, MV, Meray Sierra, R, Scarcella, S, et al. (2015) In vivo assessment of closantel ovicidal activity in Fasciola hepatica eggs. Experimental Parasitology 160, 49–53.Google Scholar
Torres, D and Espino, AM (2006) Mapping of B-cell epitopes on a novel 11.5-kilodalton Fasciola hepatica-Schistosoma mansoni cross-reactive antigen belonging to a member of the F. hepatica saposin-like protein family. Infection and Immunity 74, 4932–4938.Google Scholar
Yang, J, Jin, M, Chen, J, et al. (2007) Development and evaluation of an immunochromatographic strip for detecting of streptococcus suis type 2 antibody. Journal of Veterinary Diagnostic Investigation 19, 355–361.Google Scholar
Yu, JE, Ouh, IO, Kang, H, et al. (2018) An enhanced immunochromatographic strip test using colloidal gold nanoparticle-labeled dual-type N proteins for detection of antibodies to PRRS virus. Journal of Veterinary Science 19, 519–527.Google Scholar
Supplementary material: File

Xifeng et al. supplementary material

Figures S1-S4

Download Xifeng et al. supplementary material(File)
File 305.2 KB