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Cryopreservation of Angiostrongylus cantonensis third-stage larvae

Published online by Cambridge University Press:  05 June 2009

R. K. Mahajan  and
D. M. Renapurkar
R. K. Mahajan
Affiliation:
Department of Zoonosis, Haffkine institute, Parel Bomhay-400 012, India
D. M. Renapurkar
Affiliation:
Department of Zoonosis, Haffkine institute, Parel Bomhay-400 012, India
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Abstract

An optimum protocol for the cryopreservation of third-stage larvae (L3) of Angiostrongylus cantonensis was determined by: (i) use of different dimethyl sulphoxide (DMSO) concentrations; (ii) use of different FBS concentrations in RPMI-1640 medium; and (iii) variation in the thawing temperature. Studies showed that with Concentrations of DMSO at 4·8% and FBS at 40% and a thawing temperature of 43°C, L3 survival assessed by motility was highest at 56%. The infectivity of the cryopreserved L3 of A. cantonensis was 20% compared to controls obtained from slugs.

Keywords

Angiostrongylus cantonensislarvaecryopreservationtechniquesNematoda
Type
Research Papers
Information
Journal of Helminthology , Volume 67 , Issue 3 , September 1993 , pp. 233 - 237
Copyright
Copyright © Cambridge University Press 1993

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