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A Convenient Modification of the Dilution Hatching Trial for the Potato-root Eelworm, Heterodera rostochiensis Wollenweber

Published online by Cambridge University Press:  18 November 2009

C. Ellenby
Affiliation:
Department of Zoology, King's College, University of Durham, Newcastle-upon-Tyne
A. B. Gilbert
Affiliation:
Department of Zoology, King's College, University of Durham, Newcastle-upon-Tyne

Extract

The number of cysts used for each replicate in a dilution hatching trial may be reduced to 10. Log. total hatch is used, instead of total hatch itself, and the mean for each dilution determined from the log. values; the logs, of these means, plotted against dilution in the usual way, gives a straight line as in the original Fenwick technique. Reduction of the number of cysts permits the use of commercially available utensils to form an easily handled set-up.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1960

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References

Ellenby, C., 1943.—“A modification of the Gemmell single-cyst technique for the potato strain of the eelworm Heterodera schachtii Schmidt.” Nature, Lond., 152, 133. (W.L. 14900)CrossRefGoogle Scholar
Ellenby, C., AND Gilbert, A. B.,1957.—“Cardiotonic acticity of the potato-root eelworm hatching factor.” Nature, Lond., 180,11051106. (W.L. 14900)Google Scholar
Fenwick, D. W., 1952.—“The bio-assay of potato-root diffusate.” Ann. appl. Biol., 39, 457467. (W.L. 1025)CrossRefGoogle Scholar