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Comparative evaluation of various immunodiagnostic tests for the diagnosis of Taenia solium cysticercosis in pigs, using fractionated antigens

Published online by Cambridge University Press:  05 June 2009

D. Kumar  and
S. N. S. Gaur
D. Kumar
Affiliation:
Department of Parasitology, College of Veterinary Sciences, G. B. Pant University of Agriculture & Technology, Pantnagar 263 145, Nainital, India
S. N. S. Gaur
Affiliation:
Department of Parasitology, College of Veterinary Sciences, G. B. Pant University of Agriculture & Technology, Pantnagar 263 145, Nainital, India
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Abstract

The sensitivity and specificity of double immunodiffusion (DID), indirect haemagglutination test (IHA), immunoelectrophoresis (IEP), counterimmunoelectrophoresis (CIEP) and enzyme-linked immunosorbent assay (ELISA) were evaluated and compared using saline extracted of Taenia solium larval scolex and its Sephadex G-200 fractionated 1st and 2nd peak as antigens. Various immunodiagnostic tests gave different results with different antigens. Highest sensitivity (92·5%) was obtained with ELISA using 1st peak antigen and lowest sensitivity was obtained with DID. However, 88·4% and 84·6% sensitivity was obtained with IHA and CIEP respectively using scolex antigen. CIEP gave better results as compared to IEP. Crude antigen gave high sensitivity but less specificity. It was concluded that CIEP can be used as a field test for the anti-mortem diagnosis and ELISA can be employed for laboratory confirmation of T. solium cysticercosis in pigs using frationated 1st peak antigen.

Keywords

Taenia soliumCysticercus cellulosaepigserodiagnosis
Type
Research Article
Information
Journal of Helminthology , Volume 63 , Issue 1 , March 1989 , pp. 13 - 17
Copyright
Copyright © Cambridge University Press 1989

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References

REFERENCES

Biagi, F. F. & Tay, J. (1958) A precipitation reaction for the diagnosis of cysticercosis. American Journal of Tropical Medicine and Hygiene, 7, 63–65.CrossRefGoogle ScholarPubMed
Chematai, A. K., Bowry, T. R. & Ahmed, Z. (1981) Evaulation of five immunodiagnostic techniques in echinococcosis patients. Bulletin of the World Health Organization, 59, 767–772.Google Scholar
Dottorini, S., Tassi, C. & Baldelli, F. (1981) ELISA (Enzyme-linked immunosorbent assay) for diagnosis of human hydatid disease. Bollettino dell'istituto Sieroterapico Milanese, 60, 137–143.Google ScholarPubMed
Espinoza, B., Ruiz-pakacios, G., Tovar, A., Sandoval, M. A., Plancarte, A. & Flisser, A. (1986) Characterization by enzyme-linked immunosorbent assay of the humoral immune response in patients with neurocysticercosis and its application in immunodiagnosis. Journal of Clinical Microbiology, 24, 536–541.CrossRefGoogle ScholarPubMed
Felgner, P. (1978) Antibody activity in stick ELISA as compared to other quantitative immunological tests in sera of echinococcosis cases. Tropenmedizin und Parasitologie, 29, 417–422.Google ScholarPubMed
Garabedian, G. A., Matossian, R. M. & Djanian, A. Y. (1957) An indirect haemagglutination test for hydatid disease. Journal of Immunology, 78, 269–271.CrossRefGoogle ScholarPubMed
Geerts, S., Kumar, V. & Aerts, N. (1979) Antigenic components of Taenia saginata and their relevance to the diagnosis of bovine cysticercosis by immunoelectrophoresis. Journal of Helminthology, 53, 293–299.CrossRefGoogle Scholar
Geerts, S., Kumar, V. & Aerts, N. (1980) Rapid diagnosis of bovine cysticercosis by counter-immunoelectrophoresis. Annales de la Société Belge de Médecine Tropicale, 60, 173–182.Google Scholar
Geerts, S., Kumar, V., Aerts, N. & Ceulemans, F. (1981a) Comparative evaluation of immunoelectrophoresis, counterimmunoelectrophoresis and enzyme-linked immunosorbent assay for the diagnosis of Taenia saginata cysticercosis. Veterinary Parasitology, 8, 299–307.CrossRefGoogle Scholar
Geerts, S., Kumar, V., Ceulemans, F. & Mortelmans, J. (1981b) Serodiagnosis of Taenia saginata cysticercosis in experimentally and naturally infected cattle by enzyme-linked immunosorbent assay. Research in Veterinary Science, 30, 288–293.CrossRefGoogle ScholarPubMed
Gottstein, B., Tsang, V. C. W. & Schantz, P. M. (1986) Demonstration of species-specific and cross-reactive components of Taenia solium metacestode antigen. American Journal of Tropical Medicine and Hygiene, 35, 308–313.CrossRefGoogle Scholar
Gottstein, B., Zini, D. & Schantz, P. M. (1987) Species-specific immunodiagnosis of Taenia solium cysticercosis by ELISA and immunoblotting. Tropical Medicine and Parasitology, 38, 299–303.Google ScholarPubMed
Herbert, I. V. & Oberg, O. C. (1975) Serological studies on pigs experimentally infected with Taenia solium or Taenia hydatigena. Journal of Comparative Pathology, 85, 487–498.CrossRefGoogle ScholarPubMed
Ivanovic, M. D. (1980) Counterimmunoelectrophoresis: A method for diagnosing cerebral cysticercosis. Revista Medicina de Chile, 108, 918–920.Google Scholar
Kumar, D. & Gaur, S. N. S. (1987a) Serodiagnosis of porcine cysticercosis by enzyme-linked immunosorbent assay (ELISA) using fractionated antigens. Veterinary Parasitology, 24, 195–202.CrossRefGoogle ScholarPubMed
Kumar, D. & Gaur, S. N. S. (1987a) Counterimmunoelectrophoresis in the diagnosis of Taenia solium cysticercosis in pigs using fractionated antigens. Journal of Veterinary Parasitology, 1, 43–46.Google Scholar
Kumar, D., Gaur, S. N. S. & Pathak, K. M. L. (1987) Fractionation and characterization of cysticercus of Taenia solium. Research in Veterinary Science, 43, 395–397.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193, 265–275.CrossRefGoogle ScholarPubMed
Pathak, K. M. L., Gaur, S. N. S. & Garg, S. K. (1984) Counter-current immunoelectrophoresis, a new technique for rapid serodiagnosis of porcine cysticercosis. Journal of Helminthology, 58, 321–324.CrossRefGoogle ScholarPubMed
Proctor, E. M., Powell, S. J. & Elsdon-Dew, R. (1966) The serological diagnosis of cysticercosis. Annals of Tropical Medicine and Parasitology, 60, 146–151.CrossRefGoogle ScholarPubMed
Van tiggele, L. J. & Over, H. J. (1976) Serological diagnosis of fascioliasis. Veterinary Parasitology, 1, 239–248.CrossRefGoogle Scholar