Hostname: page-component-77c89778f8-m42fx Total loading time: 0 Render date: 2024-07-19T17:20:21.086Z Has data issue: false hasContentIssue false
  • English
  • Français

A Routine Method for the Maintenance of Schistosoma mansoni in vitro

Published online by Cambridge University Press:  18 November 2009

D. L. H. Robinson
D. L. H. Robinson
Affiliation:
From the Bilharzta Research Group of the Medical Research Council at the Winches Farm Field Station of the London School of Hygiene and Tropical Medicine
Get access Rights & Permissions [Opens in a new window]

Extract

1. The method of maintaining schistosomes in vitro is described in detail.

2. Observations made on cultured S. mansoni are given.

3. Investigation of glycogen content shows that there is no significant difference between the reserve of glycogen in fresh and cultured worms. It is suggested that the worms do not rely on their reserve of glycogen but can take up carbohydrate from the medium.

4. The method could be adapted for the culture of other helminths besides schistosomes.

Type
Research Article
Information
Journal of Helminthology , Volume 29 , Issue 4 , January 1956 , pp. 193 - 202
Copyright
Copyright © Cambridge University Press 1956

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Boettiger, E. G., 1946.— “Diphenylamine as colorimetric agent in the determination of glycogen”. J. cell. comp. Physiol., 27, 1–8. (W.L. 110986.)Google Scholar
Bueding, E., 1951.— Metabolism of Helminths. From “Parasitic Infections in Man”, ed. Most, H., p. 150. N.Y.: Columbia Univ. Press.Google Scholar
Bueding, E. and Koletsky, S., 1950.— “Content and distribution of glycogen in Schistosoma mansoni”. Proc. Soc. exp. Biol., N.Y., 73, 594–596. (W.L. 16913.)Google Scholar
Bueding, E., Peters, L. and Waite, J. F., 1947.— “Effect of 2-methyl-l, 4-naphthoquinone on glycolysis of Schistosoma mansoni”. Proc. Soc. exp. Biol., N.Y., 64, 111–113. (W.L. 16913.)CrossRefGoogle Scholar
Chu, H. J., 1938.— “Certain behaviour reactions of Schistosoma japonicum and Clonorchis sinensis in vitro”. Chin. med. J. Sappl., 2, 411–417. (W.L. 6177£.)Google Scholar
Heatley, N. G., 1935.— “The distribution of glycogen in the regions of the amphibian gastrula; with a method for the micro-determination of glycogen”. Biochem. J., 29, 2568–2572. (W.L. 2967.)CrossRefGoogle Scholar
Newsome, J. and Robinson, D. L. H., 1954.— “Investigation of Methods of Maintaining Schistosoma mansoni in vitro”. Ann. trop. Med. Parasit., 48, 194–200. (W.L. 1063.)CrossRefGoogle ScholarPubMed
Yolles, T. K., Moore, D. V., Degiusti, D. L.Ripsom, C. A. and Meleney, H. E., 1947.— “A technique for the perfusion of laboratory animals for the recovery of schistosomes”. J. Parasit., 33, 419. (W.L. 11428.)CrossRefGoogle ScholarPubMed