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An enhanced humoral immune response against the swimbladder nematode, Anguillicola crassus, in the Japanese eel, Anguilla japonica, compared with the European eel, A. anguilla

Published online by Cambridge University Press:  11 April 2024

M.E. Nielsen
M.E. Nielsen*
Affiliation:
Marine Biological Laboratory, University of Copenhagen, 5 Strandpromenaden, DK-3000 Helsingør, Denmark
*
*Address for correspondence: The Royal Veterinary and Agricultural University, Department of Veterinary Microbiology, Section of Fish Diseases, Bü lowsvej 13, DK-1870 Denmark. Fax: +45 35 28 27 11 E-mail: men@kvl.dk
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Abstract

The humoral immune response in the two eel species, Anguilla japonica and Anguilla anguilla against two fractions of antigens in Anguillicola crassus were studied.Within species, both eel species showed significantly elevated titres compared with controls when immunized with antigens from Anguillicola crassus.In interspecific comparison, Anguilla japonicashowed significantly elevated titres in comparison with Anguilla anguilla. Immunization of Anguilla anguillacaused a significantly decrease in the plasma levels of protein in comparison with control fish and all groups of Anguilla japonica. In contrast, Anguilla japonica showed significantly lower plasma levels of Ig in all groups compared with Anguilla anguilla.The different susceptibilities to Anguillicola crassus between the natural host, Anguilla japonica, and the naı¨ve, Anguilla anguilla, is partly due to differences in the ability of the two eel species to mount a humoral immune response.

Type
Research Article
Information
Journal of Helminthology , Volume 73 , Issue 3 , March 1999 , pp. 227 - 232
Copyright
Copyright © Cambridge University Press 1999

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References

Buchmann, K. & Pedersen, K. (1994) A study on teleost phylogeny using specific antisera. Journal of Fish Biology 45, 901–903.CrossRefGoogle Scholar
Buchmann, K., Pedersen, L.Ø. & Glamann, J. (1991) Humoral immune response of European eel Anguilla anguilla to a major antigen in Anguillicola crassus (Nematoda). Diseases of Aquatic Organisms 12, 55–57.CrossRefGoogle Scholar
Buchmann, K., Pedersen, L.Ø. & Glamann, J. (1992) Affinity purification of antigen-specific serum immunoglobulin from the European eel (Anguilla anguilla). Scandinavian Journal of Immunology 36, 89–97.CrossRefGoogle ScholarPubMed
DeCharleroy, D., Cannaerts, V., Augustijn, H., Grisez, L., Boon, J.H. & Ollevier, F. (1990) An improved method for artificial infection of the European eel, Anguilla anguilla, with Anguillicola crassus (Nematoda, Dracunculoidea). Journal of Applied Ichthyology 6, 182–188.CrossRefGoogle Scholar
Eddy, F.B. (1981) Effects of stress on osmotic regulation in fish. pp. 49–75 in Pickering, A.D. (Ed.) Stress and fish. Academic Press.Google Scholar
Egusa, S. (1979) Notes on the culture of the European eel (Anguilla anguilla L.) in Japanese eel farming ponds. Rapports et Procés-Verbaux des Réunions . Conseil Internationale pour l'Exploration de la Mer 174, 51–58.Google Scholar
Fries, L.T., Williams, D.J. & Johnson, S.K. (1996) Occurrence of Anguillicola crassus, an exotic parasitic swimbladder nematode of eels, in the Southeastern United States. Transactions of the American Fisheries Society 125, 794–797.2.3.CO;2>CrossRefGoogle Scholar
Haenen, O.L.M., Grisez, L. De, Charleroy, D., Belpaire, C. & Ollivier, F. (1989) Experimentally induced infections of the European eel, Anguilla anguilla, with Anguillicola crassus (Nematoda: Dracunculoidea) and subsequent migration of larvae. Diseases of Aquatic Organisms 7, 97–101.CrossRefGoogle Scholar
Haenen, O.L.M., van Wijngaarden, T.A.M., van der Heijden, M.H.T., Höglund, J., Cornelissen, J.B.J.W., van Leengoed, L.A.M.G., Borgsteede, W.B. & Muiswinkel, W.B. (1996) Effects of experimental infections with different doses of Anguillicola crassus (Nematoda: Dracunculoidea) on European eel (Anguilla anguilla). Aquaculture 141, 41–57.CrossRefGoogle Scholar
Heggberget, T.G. & Johnsen, B.O. (1982) Infestions by Gyrodactylus sp. of Atlantic salmon, Salmo salar L., in Norwegian rivers. Journal of Fish Biology 21, 15–26.CrossRefGoogle Scholar
Hoffman, G.L. (1970) Intercontinental and transcontinental dissemination and transfaunation of fish parasites with emphasis on whirling disease (Myxosoma cerebralis). pp. 69–81 in Snieszko, S.F. (Ed.) A symposium on diseases of fishes and shellfishes. Special publication No. 5. American Fisheries Society.Google Scholar
Höglund, J. & Pilström, L. (1994) Purification of adult Anguillicola crassus whole-worm extract antigens for detection of specific antibodies in serum from the European eel (Anguilla anguilla) by ELISA. Fish and Shellfish Immunology 4, 311–319.CrossRefGoogle Scholar
Höglund, J. & Pilström, L. (1995) Mechanical isolation and characterization of antigens from adult Anguillicola crassus . Fish and Shellfish Immunology 5, 51–60.CrossRefGoogle Scholar
Kuwurahara, A., Niimi, A. & Itagaki, H. (1974) Studies on a nematode parasitic in the air bladder of the eel. I. Description of Anguillicola crassa n. sp. (Philometridea, Anguillicolidae). Japanese Journal of Parasitology 23, 275–279.Google Scholar
Køie, M. (1991) Swimbladder nematodes (Anguillicola spp.) and gill monogeneans (Pseudodactylogyrus spp.) parasitic on the European eel (Anguilla anguilla) Journal du Conseil Internationale pour l'Exploration de la Mer 47, 391–398.CrossRefGoogle Scholar
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.CrossRefGoogle Scholar
Molnár, K., Sezékely, C. & Baska, F. (1991) Mass mortality of eel in lake Balaton due to Anguillicola crassus infection. Bulletin of the European Association of Fish Pathologists 11 (6), 211–212.Google Scholar
Molnár, K., Baska, F., Csaba, G., Glávits, R. & Sezékely, C. (1993) Pathological and histopathological studies of the swimbladder of eels Anguilla anguilla infected by Anguillicola crassus (Nematoda: Dracunculoidea). Diseases of Aquatic Organisms 15, 41–50.CrossRefGoogle Scholar
Molnár, K., Szakolczai, J. & Vetési, F. (1995) Histological changes in the swimbladder wall of eels due to abnormal location of adults and second stage larvae of Anguillicola crassus . Acta Veterinaria Hungarica 43, 125–137.Google ScholarPubMed
Nielsen, M.E. (1997) Infection status of the swimbladder worm Anguillicola crassus in silver stage European eel, Anguilla anguilla, from three different habitats in Danish waters. Journal of Applied Ichthyology 13, 195–196.CrossRefGoogle Scholar
Nielsen, M.E. & Buchmann, K. (1997) Glutathione-s-transferase is an important antigen in the eel parasite Anguillicola crassus . Journal of Helminthology 71, 319–324.CrossRefGoogle Scholar
Nielsen, M.E., Boesgaard, L., Sweeting, R.M., McKeown, B.A. & Rosenkilde, P. (1994) Plasma levels of lactate, potassium, glucose, cortisol, growth hormone and triiodo-L-thyronine in rainbow trout (Oncorhynchus mykiss) during exercise at various levels for 24 h. Canadian Journal of Zoology 72, 1643–1647.CrossRefGoogle Scholar
Nomoto, A., Koike, S. & Aoki, J. (1994) Tissue tropism and species of poliovirus infections. Trends in Microbiology 2, 47–51.CrossRefGoogle Scholar
Postlethwaite, E.K. & McDonald, D.G. (1995) Mechanisms of Na+and Cl- regulation in freshwater-adapted rainbow trout (Oncorhynchus mykiss) during exercise and stress. Journal of Experimental Biology 198, 295–304.CrossRefGoogle ScholarPubMed
van Banning, P. & Haenen, O.L.M. (1990) Effects of the swimbladder nematode Anguillicola crassus in the wild and farmed eel, Anguilla anguilla. pp. 317–330 in Perkins, F.O. & Cheng, T.C. (Eds) Pathology in marine science. Academic Press.Google Scholar
van der Heijden, M.H.T., Rooijakkers, J.B.M.A., Booms, G.H.R., Rombout, J.H.W.M. & Boon, J.H. (1995) Production, characterisation and applicability of monoclonal antibodies to European eel (Anguilla anguilla L., 1758) immunoglobulin. Veterinary Immunology and Immunopathology 45, 151–164.CrossRefGoogle ScholarPubMed
Wakelin, D. (1996) The immune response. pp. 11–35 in Wakelin, D. (Ed.) Immunity to parasites, 2nd edn. Cambridge University Press.Google ScholarPubMed
Yamaguti, S. (1935) Studies on the helmith fauna of Japan, part 9. Nematodes of fishes. Japanese Journal of Zoology 6, 337–386.Google Scholar