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Does rat fetal DNA induce preeclampsia in pregnant rats?

Published online by Cambridge University Press:  23 January 2015

B. Konečná ,
V. Borbélyová ,
P. Celec  and
B. Vlková
B. Konečná*
Affiliation:
Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia
V. Borbélyová
Affiliation:
Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia
P. Celec
Affiliation:
Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia Center of Molecular Medicine, Slovak Academy of Sciences, Bratislava, Slovakia Institute of Pathophysiology, Faculty of Medicine, Comenius University, Bratislava, Slovakia Department of Molecular Biology, Faculty of Medicine, Comenius University, Bratislava, Slovakia
B. Vlková
Affiliation:
Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia Center of Molecular Medicine, Slovak Academy of Sciences, Bratislava, Slovakia
*
*Address for correspondence: Barbora Vlková, MSc, PhD. Institute of Molecular Biomedicine Comenius University Sasinkova 4 811 08 Bratislava Slovakia. (Email barboravlk@gmail.com)
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Abstract

Cell-free fetal DNA in maternal circulation is higher during preeclampsia. It is unclear whether it is the cause or the consequence of the disease. The aim of this study was to prove whether injected rat fetal DNA induces preeclampsia-like symptoms in pregnant Wistar rats. They received daily i.p. injections of water or rat fetal DNA (400 μg) from gestation day 14 to 18. Blood pressure, proteinuria, placental and fetal weight were measured at gestation day 19. Plasma DNase activity, proteinuria and creatinine clearance were assessed. There was no significant difference in any of the measured parameters. The results of this study do not confirm the hypothesis that fetal DNA might induce preeclampsia. This is in contrast to others using human fetal DNA in mice. Further studies should be focused on the effects of fetal DNA from the same species protected from DNase activity.

Keywords

extracellular DNAfetal DNAinnate immunitypreeclampsia
Type
Brief Report
Information
Journal of Developmental Origins of Health and Disease , Volume 6 , Issue 1 , February 2015 , pp. 5 - 9
Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2015 

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