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Untargeted lipidomics of ovine milk to analyse the influence of different diet regimens

Published online by Cambridge University Press:  10 August 2021

Cristina Manis ,
Margherita Addis ,
Maria Sitzia ,
Paola Scano ,
Viviana Garau ,
Andrea Cabiddu ,
Marco Caredda ,
Antonio Pirisi ,
Antonio Pulina  and
Pierpaolo Roggero
...Show all authors
Cristina Manis
Affiliation:
Dipartimento di Scienze della Vita e dell'Ambiente, Cittadella Universitaria di Monserrato, 09042, Monserrato, Italy
Margherita Addis
Affiliation:
Agris Sardegna, Servizio Ricerca Prodotti di Origine Animale, Loc. Bonassai, 07040Olmedo, Italy
Maria Sitzia
Affiliation:
Agris Sardegna, Servizio Ricerca Prodotti di Origine Animale, Loc. Bonassai, 07040Olmedo, Italy
Paola Scano
Affiliation:
Dipartimento di Scienze della Vita e dell'Ambiente, Cittadella Universitaria di Monserrato, 09042, Monserrato, Italy
Viviana Garau
Affiliation:
Dipartimento di Chimica e Farmacia, Scuola di specializzazione in Farmacia Ospedaliera, Università di Sassari, via Muroni 23a, 07100, Sassari, Italy
Andrea Cabiddu
Affiliation:
Agris Sardegna, Servizio Ricerca Prodotti di Origine Animale, Loc. Bonassai, 07040Olmedo, Italy
Marco Caredda
Affiliation:
Agris Sardegna, Servizio Ricerca Prodotti di Origine Animale, Loc. Bonassai, 07040Olmedo, Italy
Antonio Pirisi
Affiliation:
Agris Sardegna, Servizio Ricerca Prodotti di Origine Animale, Loc. Bonassai, 07040Olmedo, Italy
Antonio Pulina
Affiliation:
Dipartimento di Agraria and Nucleo di Ricerca sulla Desertificazione, Università di Sassari, viale Italia 39A, 07100Sassari, Italy
Pierpaolo Roggero
Affiliation:
Dipartimento di Agraria and Nucleo di Ricerca sulla Desertificazione, Università di Sassari, viale Italia 39A, 07100Sassari, Italy
Pierluigi Caboni*
Affiliation:
Dipartimento di Scienze della Vita e dell'Ambiente, Cittadella Universitaria di Monserrato, 09042, Monserrato, Italy
*
Author for correspondence: Pierluigi Caboni, Email: caboni@unica.it
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Abstract

In this work we report a lipidomics approach to study the effects of two diet systems on the composition of ovine milk. Milk from two groups of Sarda sheep grazing on 40% (P40) and 60% (P60) of pasture were analyzed by a UHPLC-QTOF-MS analytical platform and data submitted to multivariate statistical analysis. Pairwise partial least square discriminant analysis of the lipid profile of the data was carried out to classify samples and to find discriminant lipids. The two dietary groups were characterized by differences in triacylglycerols, phosphocholines and phosphatidylethanolamines levels. Discriminants of the P40 group were TG and PC containing in their backbone saturated medium chain FA thus suggesting greater de novo fatty synthesis in the mammary gland. On the other hand, the P60 group was characterized by TG and PC formed by unsaturated long chain FA originating from the diet or from lipid mobilization.

Keywords

Animal diet managementdiscriminant analysislipidomicsmass spectrometry
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 3 , August 2021 , pp. 261 - 264
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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