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Transcripts and protein levels of CSN1S1 and CSN3 genes in dairy cattle mammary gland secretory tissue during chronic staphylococcal infection

Published online by Cambridge University Press:  05 March 2021

Ewelina Kawecka-Grochocka ,
Magdalena Zalewska ,
Aleksandra Kapusta ,
Tomasz Ząbek ,
Magdalena Rzewuska ,
Sławomir Petrykowski  and
Emilia Bagnicka
Ewelina Kawecka-Grochocka
Affiliation:
Department of Biotechnology and Nutrigenomics, Institute of Genetics and Biotechnology, Polish Academy of Sciences, 36A Postepu St., Jastrzębiec, Poland Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 8 Ciszewskiego St., Warsaw, Poland
Magdalena Zalewska
Affiliation:
Department of Applied Microbiology, Faculty of Biology, University of Warsaw, Institute of Microbiology, 1 Miecznikowa St., Warsaw, Poland
Aleksandra Kapusta
Affiliation:
Department of Biotechnology and Nutrigenomics, Institute of Genetics and Biotechnology, Polish Academy of Sciences, 36A Postepu St., Jastrzębiec, Poland
Tomasz Ząbek
Affiliation:
Department of Animal Molecular Biology, The National Research Institute of Animal Production, 1 Krakowska St., Balice near Krakow, Poland
Magdalena Rzewuska
Affiliation:
Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 8 Ciszewskiego St., Warsaw, Poland
Sławomir Petrykowski
Affiliation:
Experimental Farm, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, 36A Postepu St., Jastrzębiec, Poland
Emilia Bagnicka*
Affiliation:
Department of Biotechnology and Nutrigenomics, Institute of Genetics and Biotechnology, Polish Academy of Sciences, 36A Postepu St., Jastrzębiec, Poland
*
Author for correspondence: Emilia Bagnicka, Email: e.bagnicka@igbzpan.pl
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Abstract

Our objective was to determine the influence of chronic coagulase-positive staphylococci (CoPS) or coagulase-negative staphylococci (CoNS) infection on the mRNA and protein levels of two main milk proteins responsible for cheese curd quantity and quality, alpha-S1-casein (CSN1S1) and kappa-casein (CSN3). Measurements were made in cow mammary parenchyma with a prevalence of secretory tissue (MGST). Samples of MGST were collected from the separate quarters and divided into CoPS, CoNS and bacteria-free (H) groups according to the microbiological status of the quarter milk. No differences in CSN1S1 and CSN3 mRNA level were found between groups, however, CSN1S1 protein level was significantly higher in the H group than the CoNS group, and CSN3 protein level was significantly higher in H than CoPS group. Hence, while the CSN1S1 and CSN3 genes appear to be constitutively expressed at the mRNA level in dairy cow MGST during mastitis, CoNS infection negatively affected CSN1S1 protein level, and CoPS infection negatively affected CSN3 protein level. The lack of change at the mRNA level suggests that staphylococcal infection may affect the post-transcriptional or post-translational modifications.

Keywords

Dairy cattleCSN1S1CSN3inflammationmammary glandstaphylococci
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 1 , February 2021 , pp. 73 - 77
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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