Hostname: page-component-848d4c4894-sjtt6 Total loading time: 0 Render date: 2024-07-07T10:38:11.611Z Has data issue: false hasContentIssue false
  • English
  • Français

Separation and quantification of milk casein from different buffalo breeds

Published online by Cambridge University Press:  07 September 2016

Shanshan Li ,
Ling Li ,
Qingkun Zeng ,
Jianxin Liu  and
Daxi Ren
Shanshan Li
Affiliation:
Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310029, People's Republic of China
Ling Li
Affiliation:
Water Buffalo Institute, Chinese Academy of Agricultural Science, Nanning 530001, People's Republic of China Guangxi Key Laboratory of Buffalo Genetics, Reproduction and Breeding, Nanning 530001, People's Republic of China
Qingkun Zeng
Affiliation:
Water Buffalo Institute, Chinese Academy of Agricultural Science, Nanning 530001, People's Republic of China
Jianxin Liu
Affiliation:
Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310029, People's Republic of China
Daxi Ren*
Affiliation:
Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310029, People's Republic of China
*
*For correspondence; e-mail: dxren@zju.edu.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

Understanding the milk protein expression profile in different buffalo breeds plays an important role in improving hybrid selection and determining the effects on milk protein synthesis. The aim of this research is to compare the differences in milk protein content, composition and distribution between River buffalo and their crossbreeds for hybrid screening. Four groups of milk samples that included Nili-Ravi (N), Murrah (M), a Nili-Ravi-Murrah crossbreed (M-N), and a crossbreed of river buffalo with local swamp buffalo (C) were collected. The protein composition of the buffalo milk was determined by RP-HPLC. A gel-based proteomic approach consisting of two-dimensional gel electrophoresis coupled with mass spectrometry was utilised for the detailed protein characterisation of milk from different breeds. The results of this analysis showed that the river/swamp buffalo crossbreed (C) displayed the highest content of total protein (4·46%) and κ-casein (11·14%) but the lowest content of α-lactalbumin (6·79%). By selecting 23 different protein spots among the four types of milk that contained the most spots corresponding to κ-casein, β-casein and αs1-casein, correlations between the crossbreeds, protein polymorphism and phosphorylation could be made. The results of this study indicate that crossbreeding a swamp buffalo with a river buffalo has a notable effect on the protein content and composition that may be exploited for producing high-quality raw milk in food technology applications and dairy food production.

Keywords

Swamp buffaloriver buffalocrossbreedmilk proteincomparative proteomics
Type
Research Article
Information
Journal of Dairy Research , Volume 83 , Issue 3 , August 2016 , pp. 317 - 325
Copyright
Copyright © Proprietors of Journal of Dairy Research 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Addeo, F, Mercier, JC & Ribadeau-Dumas, B 1977 The caseins of buffalo milk. Journal of Dairy Research 44 455–468 Google Scholar
Ageitos, JM, Vallejo, JA, Poza, M & Villa, TG 2006 Fluorescein thiocarbamoyl-kappa-casein assay for the specific testing of milk-clotting proteases. Journal of Dairy Science 89 3770–3777 CrossRefGoogle ScholarPubMed
Aggarwal, RAK, Sodhi, M, Mukesh, M, Ahlawat, SPS, Kumar, Y, Chakra-Vorty, AK & Raina, VS 2007 Kappa casein polymorphism and milk traits in Indian Murrah buffalo (Bubalus bubalis). Milchwissenschaft – Milk Science International 62 119–121 Google Scholar
Beck, KL, Weber, D, Phinney, BS, Smilowitz, JT, Hinde, K, Lonnerdal, B, Korf, I & Lemay, DG 2015 Comparative proteomics of human and macaque milk reveals species-specific nutrition during postnatal development. Journal of Proteome Research 14 2143–2157 Google Scholar
Bionaz, M & Loor, JJ 2011 Gene networks driving bovine mammary protein synthesis during the lactation cycle. Bioinformatics Biology Insights 5 83–98 CrossRefGoogle ScholarPubMed
Bobe, G, Beitz, DC, Freeman, AE & Lindberg, GL 1998 Separation and quantification of bovine milk proteins by reversed-phase high-performance liquid chromatography. Journal of Agricultural and Food Chemistry 46 458–463 Google Scholar
Bonfatti, V, Gervaso, M, Coletta, A & Carnier, P 2012 Effect of parity, days in milk, and milk yield on detailed milk protein composition in Mediterranean water buffalo. Journal of Dairy Science 95 4223–4229 Google Scholar
Bonfatti, V, Grigoletto, L, Cecchinato, A, Gallo, L & Carnier, P 2008 Validation of a new reversed-phase high-performance liquid chromatography method for separation and quantification of bovine milk protein genetic variants. Journal of Chromatography A 1195 101–106 CrossRefGoogle ScholarPubMed
Bonfatti, V, Giantin, M, Rostellato, R, Dacasto, M & Carnier, P 2013 Separation and quantification of water buffalo milk protein fractions and genetic variants by RP-HPLC. Food Chemistry 136 364–367 Google Scholar
Caroli, AM, Chessa, S & Erhardt, GJ 2009 Invited review: milk protein polymorphisms in cattle: effect on animal breeding and human nutrition. Journal of Dairy Science 92 5335–5352 Google Scholar
Chevalier, F & Kelly, AL 2010 Proteomic quantification of disulfide-linked polymers in raw and heated bovine milk. Journal of Agricultural and Food Chemistry 58 7437–7444 Google Scholar
Chianese, L, Caira, S, Lilla, S, Pizzolongo, F, Ferranti, P, Pugliano, G & Addeo, F 2004 Primary structure of water buffalo alpha-lactalbumin variants A and B. Journal of Dairy Research 71 14–19 Google Scholar
Chianese, L, Quarto, M, Pizzolongo, F, Calabrese, MG, Caira, S, Mauriello, R, De Pascale, S & Addeo, F 2009 Occurrence of genetic polymorphism at the alpha(s1)-casein locus in Mediterranean water buffalo milk. International Dairy Journal 19 181–189 Google Scholar
Comin, A, Cassandro, M, Chessa, S, Ojala, M, Dal Zotto, R, De Marchi, M, Carnier, P, Gallo, L, Pagnacco, G & Bittante, G 2008 Effects of composite beta- and K-casein genotypes on milk coagulation, quality, and yield traits in Italian Holstein cows. Journal of Dairy Science 91 4022–4027 Google Scholar
Cottenet, G, Blancpain, C & Golay, PA 2011 Simultaneous detection of cow and buffalo species in milk from China, India, and Pakistan using multiplex real-time PCR. Journal of Dairy Science 94 3787–3793 Google Scholar
D'Ambrosio, C, Arena, S, Salzano, AM, Renzone, G, Ledda, L & Scaloni, A 2008 A proteomic characterization of water buffalo milk fractions describing PTM of major species and the identification of minor components involved in nutrient delivery and defense against pathogens. Proteomics 8 3657–3666 Google Scholar
De Groot, N, Van Kuik-Romeijn, P, Lee, SH & De Boer, HA 2000 Increased immunoglobulin A levels in milk by over-expressing the murine polymeric immunoglobulin receptor gene in the mammary gland epithelial cells of transgenic mice. Immunology 101 218–224 Google Scholar
Farrell, HM, Jimenez-Flores, R, Bleck, GT, Brown, EM, Butler, JE, Creamer, LK, Hicks, CL, Hollar, CM, Ng-Kwai-Hang, KF & Swaisgood, HE 2004 Nomenclature of the proteins of cows’ milk - Sixth revision. Journal of Dairy Science 87 1641–1674 Google Scholar
Feligini, M, Bonizzi, I, Buffoni, JN, Cosenza, G & Ramunno, L 2009 Identification and quantification of alpha(S1), alpha(S2), beta, and kappa-Caseins in Water Buffalo milk by reverse phase-high performance liquid chromatography and mass spectrometry. Journal of Agricultural and Food Chemistry 57 2988–2992 Google Scholar
Ferranti, P, Scaloni, A, Caira, S, Chianese, L, Malorni, A & Addeo, F 1998 The primary structure of water buffalo alpha(s1)- and beta-casein: identification of phosphorylation sites and characterization of a novel beta-casein variant. Journal of Protein Chemistry 17 835–844 Google Scholar
Goncalves Lda, R, Soares, MR, Nogueira, FC, Garcia, C, Camisasca, DR, Domont, G, Feitosa, AC, Pereira Dde, A, Zingali, RB & Alves, G 2010 Comparative proteomic analysis of whole saliva from chronic periodontitis patients. Journal of Proteomics 73 1334–1341 Google Scholar
Han, BZ, Meng, Y, Li, M, Yang, YX, Ren, FZ, Zeng, QK & Nout, MJR 2007 A survey on the micro biological and chemical composition of buffalo milk in China. Food Control 18 742–746 Google Scholar
Hinz, K, O'Connor, PM, Huppertz, T, Ross, RP & Kelly, AL 2012 Comparison of the principal proteins in bovine, caprine, buffalo, equine and camel milk. Journal of Dairy Research 79 185–191 Google Scholar
Holland, JW, Gupta, R, Deeth, HC & Alewood, PF 2011 Proteomic analysis of temperature-dependent changes in stored UHT milk. Journal of Agricultural and Food Chemistry 59 1837–1846 CrossRefGoogle ScholarPubMed
Hussain, I, Bell, AE & Grandison, AS 2011 Comparison of the rheology of mozzarella-type curd made from buffalo and cows’ milk. Food Chemistry 128 500–504 Google Scholar
International Dairy Federation 2010 The world dairy situation 2010. Bulletin No. 446/2010Google Scholar
Islam, MA, Alam, MK, Islam, MN, Khan, MAS, Ekeberg, D, Rukke, EO & Vegarud, GE 2014 Principal milk components in buffalo, Holstein Cross, Indigenous cattle and red Chittagong cattle from Bangladesh. Asian-Australasian Journal of Animal Sciences 27 886–897 Google Scholar
Jensen, HB, Holland, JW, Poulsen, NA & Larsen, LB 2012 Milk protein genetic variants and isoforms identified in bovine milk representing extremes in coagulation properties. Journal of Dairy Science 95 2891–2903 Google Scholar
Kaetzel, CS 2005 The polymeric immunoglobulin receptor: bridging innate and adaptive immune responses at mucosal surfaces. Immunological Reviews 206 83–99 Google Scholar
Khedkar, CD, Kalyankar, SD & Deosarkar, SS 2016 Encyclopedia of Food and Health: Buffalo milk , 1st edition, pp. 522–528 (Ed. Caballero, B, Finglas, P & Toldrá, F). Elsevier Google Scholar
Mamone, G, Caira, S, Garro, G, Nicolai, A, Ferranti, P, Picariello, G, Malorni, A, Chianese, L & Addeo, F 2003 Casein phosphoproteome: identification of phosphoproteins by combined mass spectrometry and two-dimensional gel electrophoresis. Electrophoresis 24 2824–2837 Google Scholar
Menard, O, Ahmad, S, Rousseau, F, Briard-Bion, V, Gaucheron, F & Lopez, C 2010 Buffalo vs. cow milk fat globules: size distribution, zeta-potential, compositions in total fatty acids and in polar lipids from the milk fat globule membrane. Food Chemistry 120 544–551 Google Scholar
Monteiro, RC & Van De Winkel, JGJ 2003 IgA Fc receptors. Annual Review of Immunology 21 177–204 Google Scholar
Nanda, AS & Nakao, T 2003 Role of buffalo in the socioeconomic development of rural Asia: current status and future prospectus. Animal Science Journal 74 443–455 Google Scholar
Patel, RK, Chauhan, JB, Singh, KM & Soni, KJ 2007 Genotype and allele frequencies of κ casein and β lac toglobulin in Indian river buffalo bulls (Bubalus bubalis). Buffalo Bull 26 63–66 Google Scholar
Perna, A, Intaglietta, I, Simonetti, A & Gambacorta, E 2013 Effect of genetic type and casein haplotype on antioxidant activity of yogurts during storage. Journal of Dairy Science 96 3435–3441 Google Scholar
Ren, DX, Miao, SY, Chen, YL, Zou, CX, Liang, XW & Liu, JX 2011 Genotyping of the k-casein and beta-lactoglobulin genes in Chinese Holstein, Jersey and water buffalo by PCR-RFLP. Journal of Genetics 90 E1–E5 Google Scholar
Ren, DX, Chen, B, Chen, YL, Miao, SY & Liu, JX 2013 The effects of κ-casein polymorphism on the texture and functional properties of mozzarella cheese. International Dairy Journal 31 65–69 Google Scholar
Ren, DX, Zou, CX, Lin, B, Chen, YL, Liang, XW & Liu, JX 2015 A Comparison of Milk Protein, Amino Acid and Fatty Acid Profiles of River Buffalo and Their F1 and F2 Hybrids with Swamp Buffalo in China. Pakistan Journal of Zoology 47 1459–1465 Google Scholar
Sanchez-Macias, D, Morales-delaNuez, A, Torres, A, Hernandez-Castellano, LE, Jimenez-Flores, R, Castro, N & Arguello, A 2013 Effects of addition of somatic cells to caprine milk on cheese quality. International Dairy Journal 29 61–67 CrossRefGoogle Scholar
Shimobayashi, M & Hall, MN 2014 Making new contacts: the mTOR network in metabolism and signalling crosstalk. Nature Reviews Molecular Cell Biology 15 155–162 Google Scholar
Yang, YX, Wang, JQ, Yuan, TJ, Bu, DP, Yang, JH, Sun, P & Zhou, LY 2013 Effects of duodenal infusion of free α-linolenic acid on the plasma and milk proteome of lactating dairy cows. Animal 7 293–299 Google Scholar
Yang, YX, Zheng, N, Zhao, XW, Zhang, YD, Han, RW, Ma, L, Zhao, SG, Li, SL, Guo, TJ & Wang, JQ 2015 Proteomic characterization and comparison of mammalian milk fat globule proteomes by iTRAQ analysis. Journal of Proteomics 116 34–43 CrossRefGoogle ScholarPubMed