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Safety Assessment of Lactobacillus fermentum CECT5716, a probiotic strain isolated from human milk

Published online by Cambridge University Press:  13 March 2009

Federico Lara-Villoslada ,
Saleta Sierra ,
María Paz Díaz-Ropero ,
Juan Miguel Rodríguez ,
Jordi Xaus  and
Mónica Olivares
Federico Lara-Villoslada*
Affiliation:
Department of Nutrition and Health, Puleva Biotech S.A. Cno. Purchil n°66, 18004 Granada (Spain)
Saleta Sierra
Affiliation:
Department of Nutrition and Health, Puleva Biotech S.A. Cno. Purchil n°66, 18004 Granada (Spain)
María Paz Díaz-Ropero
Affiliation:
Department of Nutrition and Health, Puleva Biotech S.A. Cno. Purchil n°66, 18004 Granada (Spain)
Juan Miguel Rodríguez
Affiliation:
Department of Nutrition and Food Science, Universidad Complutense, Madrid (Spain)
Jordi Xaus
Affiliation:
Department of Nutrition and Health, Puleva Biotech S.A. Cno. Purchil n°66, 18004 Granada (Spain)
Mónica Olivares
Affiliation:
Department of Nutrition and Health, Puleva Biotech S.A. Cno. Purchil n°66, 18004 Granada (Spain)
*
*For correspondence; e-mail: flara@pulevabiotech.es
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Abstract

Lactobacillus fermentum CECT5716, a probiotic strain isolated from human milk, was characterized in a previous study. The objective of this study was to evaluate its sensitivity to antibiotics and its potential toxicity and translocation ability after oral administration to mice. For this puropose, 40 Balb/C mice were divided in two groups (n=20 per group). One group was treated orally with 1010 colony forming units (cfu)/mouse/day of Lb. fermentum CECT5716 during 28 d. The other group only received the excipient and was used as control. Food intake, body weight, bacterial translocation and different biochemical and haematological parameters were analysed. Oral administration of Lb. fermentum CECT5716 to mice had no adverse effects on mice. There were no significant differences in body weight or food intake between control and probiotic-treated mice. No bacteraemia was observed and there was no treatment-associated bacterial translocation to liver or spleen. Stress oxidative markers were not different in control and probiotic-treated mice. These results suggest that the strain Lb. fermentum CECT5716 is non-pathogenic for mice even in doses 10,000 times higher (expressed per kg of body weight) than those normally consumed by humans.

Keywords

Lactobacillus fermentumprobiotictranslocationtoxicitysafetymice
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 2 , May 2009 , pp. 216 - 221
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

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References

Aguirre, M & Collins, MD 1993 Lactic acid bacteria and human clinical infection. Journal of Applied Bacteriology 75 95–107CrossRefGoogle ScholarPubMed
Ballongue, J 1998 Bifidobacteria and probiotic action. In: Lactic Acid Bacteria-Microbiology and functional aspects. 2nd edition. (Eds Salminen, S & Von Wright, A). Marcel Decker, New York, p. 519Google Scholar
Bayer, AS, Chow, AW, Betts, D & Guze, LB 1978 Lactobacillemia-report of nine cases, important clinical and therapeutical considerations. American Journal of Medicine 64 808–813CrossRefGoogle Scholar
Collins, JK, Thorton, G & Sullivan, GO 1998 Selection of probiotic strains for human applications. International Dairy Journal 8 487–490CrossRefGoogle Scholar
Conway, PL 1996 Selection criteria for probiotic microorganisms. Asia and Pacific Journal of Clinical Nutrition 5 10–14Google ScholarPubMed
Dofferhoff, AS, Bom, VJ, de Vries-Hospers, HG, van Ingen, J, vd Meer, J, Hazenberg, BP, Mulder, PO & Weits, J 1992 Patterns of cytokines, plasma endotoxin, plasminogen, and acute-phase proteins during the treatment of severe sepsis in humans. Critical Care Medicine 20 185–192CrossRefGoogle ScholarPubMed
Donohue, DC, Deighton, M, Ahokas, JT & Salminen, S 1993 Toxicity of Lactic Acid Bacteria. In: Lactic acid bacteria, (Eds Salminen, S & von Wright, A). Marcel Dekker, New York, p. 639Google Scholar
EFSA 2007 Introduction of a Qualified Presumption of Safety approach for assessment of selected microorganisms referred to EFSA. Opinion of the Scientific Committee. The EFSA Journal 587 1–16Google Scholar
EFSA 2008 Update of the criteria used in the assessment of bacterial resistance to antibiotics of human or veterinary importance. The EFSA Journal 732 1–15Google Scholar
Fukunaga, K, Yoshida, M & Nakazono, N 1998 A simple, rapid, high sensitive and reproducible method quantification for plasma malondialdehyde by high performance liquid chromatography. Biomedical Chromatography 12 300–3033.0.CO;2-#>CrossRefGoogle ScholarPubMed
Furrie, E 2005 Probiotics and allergy. Proceeding of the Nutrition Society 64 465–469CrossRefGoogle ScholarPubMed
Gasser, F 1994 Safety of lactic acid bacteria and their occurrence in human clinical infections. Bulletin of the Pasteur Institute 92 45–67Google Scholar
Gil, L, Siems, W, Mazurek, B, Gross, J, Schroeder, P, Voss, P & Grune, T 2006 Age-associated analysis of oxidative stress parameters in human plasma and erythrocytes. Free Radical Research 40 495–505CrossRefGoogle Scholar
Isolauri, E, Juntunen, M, Rautanen, T, Sillanauke, P & Koiuva, T 1991 A human Lactobacillus strain (Lactobacillus casei sp strain GG) promotes recovery from acute diarrhea in children. Pediatrics 88 90–97Google ScholarPubMed
Joint FAO/WHO Working Group 2002 Report on Drafting Guidelines for the Evaluation of Probiotics in Food. Report on Drafting Guidelines for the Evaluation of Probiotics in Food. London, Ontario, Canada, p. 5 [http://www.who.int/foodsafety/publications/fs_management/en/probiotics.pdf]Google Scholar
Klare, I, Konstabel, C, Müller-Bertling, S, Reissbrodt, R, Huys, G, Vancanneyt, M, Swings, J, Goossens, H & Witte, W 2005 Evaluation of new broth media for microdilution antibiotic susceptibility testing of lactobacilli, pediococci, lactococci, and bifidobacteria. Applied and Environmental Microbiology 71 8982–8986CrossRefGoogle ScholarPubMed
Klare, I, Konstabel, C, Werner, G, Huys, G, Vankerckhoven, V, Kahlmeter, G, Hildebrandt, B, Müller-Bertling, S, Witte, W & Goossens, H 2007 Antimicrobial susceptibilities of Lactobacillus, Pediococcus and Lactococcus human isolates and cultures intended for probiotic or nutritional use. Journal of Antimicrobial Chemotherapy 59 900–912CrossRefGoogle ScholarPubMed
Land, MH, Rouster-Stevens, K, Woods, CR, Cannon, ML & Cnota, J & Shetty, AK 2005 Lactobacillus sepsis associated with probiotic therapy. Pediatrics 115 178–181CrossRefGoogle ScholarPubMed
Ma, L, Deitich, E, Specian, R, Steffen, E & Berg, E 1990 Translocation of Lactobacillus murinus from the gastrointestinal tract. Current Microbiology 80 177–184CrossRefGoogle Scholar
Malmezat, T, Breuillé, D, Pouyet, C, Mirand, PP & Obled, C 1998 Metabolism of cysteine is modified during the acute phase of sepsis in rats. Journal of Nutrition 128 97–105CrossRefGoogle ScholarPubMed
Martín, R, Langa, S, Reviriero, C, Jiménez, E, Marín, ML, Xaus, J, Fernández, L & Rodriguez, JM 2003 Human milk is a source of lactic acid bacteria for the infant gut. Journal of Pediatrics 143 754–758CrossRefGoogle ScholarPubMed
Martín, R, Olivares, M, Marín, ML, Fernández, L, Xaus, J & Rodriguez, JM 2005 Probiotic potential of 3 Lactobacilli strains isolated from breast milk. Journal of Human Lactation 21 8–17CrossRefGoogle ScholarPubMed
Olivares, M, Díaz-Ropero, MP, Gómez, N, Lara-Villoslada, F, Maldonado, JA, Martín, R, López-Huertas, E, Rodriguez, JM & Xaus, J 2006 Oral administration of two probiotic strains, Lactobacillus coryniformis CECT5711 and Lactobacillus gasseri CECT5714, enhances the intestinal function of healthy adults. International Journal of Food Microbiology 107 104–111CrossRefGoogle ScholarPubMed
Peran, L, Camuesco, D, Comalada, M, Nieto, A, Concha, A, Díaz-Ropero, MP, Olivares, M, Xaus, J, Zarzuelo, A & Galvez, J 2005 Preventive effects of a probiotic, Lactobacillus salivarius ssp. Salivarius, in the TNBS model of rat colitis. World Journal of Gastroenterology 11 5185–5192Google ScholarPubMed
Rautio, M, Jousimies-Somer, H, Kauma, H, Pietarinen, I, Saxelin, M, Tynkkynen, S, Koskela, M 1999 Liver abscess due to a Lactobacillus rhamnosus strain indistinguishable from L-rhamnosus strain GG. Clinical Infectious Diseases 28(5) 1159–1160CrossRefGoogle ScholarPubMed
Schrezenmeir, J & de Vrese, M 2001 Probiotics, prebiotics ans symbiotics–approaching a definition. American Journal of Clinical Nutrition 73 361S–364SCrossRefGoogle Scholar
Steffen, EK & Berg, RD 1983 Relationship between caecal population levels of indigenous bacteria and translocation to the mesenteric lymph nodes. Infection and Immunity 39 1215–1259CrossRefGoogle Scholar
Stine, KE & Brown, TM 1996 Measuring toxicity and assessing risk. In: Principles of Toxicology, (Eds Stine, KE & Brown, TM) CRC Lewis Publishers, FL, pp. 1–10Google Scholar
Wassenaar, TM & Klein, G 2008 Safety aspects and implications of regulation of probiotic bacteria in food and food supplements. Journal of Food Protection 71 1734–1741CrossRefGoogle ScholarPubMed
Zhou, JS, Shu, Q, Rutherfurd, KJ, Prasad, J, Gopal, KP & Gill, HS 2000 Acute oral toxicity and bacterial translocation studies on potentially probiotic strains of Lactic Acid Bacteria. Food and Chemical Toxicology 38 153–161CrossRefGoogle ScholarPubMed