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N-acetyl -β-D-glucosaminidase activity in cow milk as an indicator of mastitis

Published online by Cambridge University Press:  23 May 2016

Mari Hovinen ,
Heli Simojoki ,
Reeta Pösö ,
Jenni Suolaniemi ,
Piret Kalmus ,
Leena Suojala  and
Satu Pyörälä
Mari Hovinen*
Affiliation:
Department of Production Animal Medicine, University of Helsinki, Helsinki, Finland
Heli Simojoki
Affiliation:
Department of Production Animal Medicine, University of Helsinki, Helsinki, Finland
Reeta Pösö
Affiliation:
Department of Veterinary Biosciences, University of Helsinki, Helsinki, Finland
Jenni Suolaniemi
Affiliation:
Department of Production Animal Medicine, University of Helsinki, Helsinki, Finland
Piret Kalmus
Affiliation:
Department of Clinical Veterinary Medicine, Estonian University of Life Sciences, Estonia
Leena Suojala
Affiliation:
Central Union of Agricultural Producers and Forest Owners MTK, Helsinki, Finland
Satu Pyörälä
Affiliation:
Department of Production Animal Medicine, University of Helsinki, Helsinki, Finland
*
*For correspondence; e-mail: mari.hovinen@helsinki.fi
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Abstract

Activity of lysosomal N-acetyl-β-d-glucosaminidase (NAGase) in milk has been used as an indicator of bovine mastitis. We studied NAGase activity of 808 milk samples from healthy quarters and quarters of cows with spontaneous subclinical and clinical mastitis. Associations between milk NAGase activity and milk somatic cell count (SCC), mastitis causing pathogen, quarter, parity, days in milk (DIM) and season were studied. In addition, the performance of NAGase activity in detecting clinical and subclinical mastitis and distinguishing infections caused by minor and major bacteria was investigated. Our results indicate that NAGase activity can be used to detect both subclinical and clinical mastitis with a high level of accuracy (0·85 and 0·99). Incomplete correlation between NAGase activity and SCC suggests that a substantial proportion of NAGase activity comes from damaged epithelial cells of the udder in addition to somatic cells. We therefore recommend determination of NAGase activity from quarter foremilk after at least six hours from the last milking using the method described. Samples should be frozen before analysis. NAGase activity should be interpreted according to DIM, at least during the first month of lactation. Based on the results of the present study, a reference value for normal milk NAGase activity of 0·1–1·04 pmoles 4-MU/min/μl for cows with ≥30 DIM (196 samples) could be proposed. We consider milk NAGase activity to be an accurate indicator of subclinical and clinical mastitis.

Keywords

N-acetyl -β-d-glucosaminidasecow milkindicator of mastitis
Type
Research Article
Information
Journal of Dairy Research , Volume 83 , Issue 2 , May 2016 , pp. 219 - 227
Copyright
Copyright © Proprietors of Journal of Dairy Research 2016 

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