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Low resolution NMR spectroscopy: a tool to study protein denaturation: I. Application to diamagnetic whey proteins

Published online by Cambridge University Press:  01 June 2009

Pierre Lambelet ,
Rafael Berrocal  and
Francine Ducret
Pierre Lambelet
Affiliation:
Nestec Ltd, Nestlé Research Centre, Vers-chez-les-Blanc, CH-1000 Lausanne, 26, Switzerland
Rafael Berrocal
Affiliation:
Nestec Ltd, Nestlé Research Centre, Vers-chez-les-Blanc, CH-1000 Lausanne, 26, Switzerland
Francine Ducret
Affiliation:
Nestec Ltd, Nestlé Research Centre, Vers-chez-les-Blanc, CH-1000 Lausanne, 26, Switzerland
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Summary

A method using low resolution NMR spectroscopy is described for investigating whey protein thermal denaturation. The method is based on measuring at 20 °C changes in water proton transverse (T2) relaxation parameter following the denaturing treatment. This parameter is shown to be sensitive to protein denaturation and not to other phenomena such as gelation. Examples are given for the qualitative study of protein thermal denaturation in whey protein concentratc, β-lactoglobulin, α-lactalbumin, bovine serum albumin and immunoglobulins aqueous solutions and for the quantitative determination of thermal denaturation in whey protein concentrate solutions.

Type
Original Articles
Information
Journal of Dairy Research , Volume 56 , Issue 2 , May 1989 , pp. 211 - 222
Copyright
Copyright © Proprietors of Journal of Dairy Research 1989

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