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Localization of glycosylated κ-casein in bovine casein micelles by lectin-labelled gold granules

Published online by Cambridge University Press:  01 June 2009

Marc Horisberger  and
Monique Vonlanthen
Marc Horisberger
Affiliation:
Nestlé Research Department, CH-1814 La Tour-de-Peilz, Switzerland
Monique Vonlanthen
Affiliation:
Nestlé Research Department, CH-1814 La Tour-de-Peilz, Switzerland
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Summary

The localization of glycosylated k-casein in isolated bovine casein micelles was investigated at the ultrastructural level with gold granules labelled with the Ricinus communis lectin specific for galactose. No evidence was obtained for the presence of glycosylated k-casein on the surface of glutaraldehyde-fixed micelles whether or not they had been treated with neuraminidase. Glycosylated k-casein was mainly located in the bridging network interconnecting the micelles and appeared to be loosely associated with the micelles. When thin sections of micelles were marked, no clear-cut evidence was observed for the presence of intramicellar glycosylated k-casein.

Type
Original Articles
Information
Journal of Dairy Research , Volume 47 , Issue 2 , June 1980 , pp. 185 - 191
Copyright
Copyright © Proprietors of Journal of Dairy Research 1980

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