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Isolation, preparation and the amino acid composition of 4 milk-fat globule membrane proteins solubilized by treatment with sodium dodecyl sulphate

Published online by Cambridge University Press:  01 June 2009

T. E. Cawston ,
M. Anderson  and
G. C. Cheeseman
T. E. Cawston
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, RG2 9AT
M. Anderson
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, RG2 9AT
G. C. Cheeseman
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, RG2 9AT
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Summary

Milk-fat globule membrane (MFGM) proteins were solubilized by treatment with SDS. Four of the major proteins were isolated as SDS complexes using column chromatography. The purity of each isolate was determined by SDS polyacrylamide gel electrophoresis and sufficient of each protein was obtained for amino acid analysis. The amino acid compositions of the isolated MFGM proteins and a total MFGM protein extract were determined. Differences in amino acid composition were found in particular between the major MFGM glycoprotein and the other 3 membrane proteins. The relationships of the amino acid composition to protein properties and structure are discussed.

Type
Original Articles
Information
Journal of Dairy Research , Volume 43 , Issue 3 , October 1976 , pp. 401 - 409
Copyright
Copyright © Proprietors of Journal of Dairy Research 1976

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References

REFERENCES

Anderson, M. & Brooker, B. E. (1975). Journal of Dairy Science 58, 1442.CrossRefGoogle Scholar
Anderson, M., Cawston, T. & Cheeseman, G. C. (1974). Biochemical Journal 139, 653.Google Scholar
Anderson, M. & Cheeseman, G. C. (1971). Journal of Dairy Research 38, 409.CrossRefGoogle Scholar
Anderson, M., Cheeseman, G. C., Knight, D. J. & Shipe, W. F. (1972). Journal of Dairy Research 39, 95.Google Scholar
Bargmann, W. & Knoop, A. (1959). Zeitschrift für Zellforschung und Mikroskopische Anatomie 49, 344.Google Scholar
Capaldi, R. A. & Vanderkooi, G. (1972). Proceedings of the National Academy of Sciences of the United States of America 69, 930.Google Scholar
Cawston, T. E. (1974). Thesis, University of Reading.Google Scholar
Chien, H. C. & Richardson, T. (1967). Journal of Dairy Science 50, 1868.Google Scholar
Jackson, R. H., Coulson, E. J. & Clark, W. R. (1962). Archives of Biochemistry and Biophysics 97, 373.Google Scholar
Keenan, T. W. & Huang, C. M. (1972). Journal of Dairy Science 55, 1586.Google Scholar
Keenan, T. W., MorrÉ, D. J., Olson, D. E., Yunghans, W. N. & Patton, S. (1970). Journal of Cell Biology 44, 80.Google Scholar
Kitchen, B. J. (1974). Biochimica et Biophysica Acta 356, 257.Google Scholar
Knoop, A. (1962) cited by Brunner, J. R. (1974) in Fundamentals of Dairy Chemistry, 2nd edn, p. 548. (Eds B. H. Webb, A. H. Johnson and J. A. Alford.) Westport, Conn.: Avi Pub. Co.Google Scholar
Kobylka, D. & Carraway, K. L. (1972). Biochimica et Biophysica Acta 288, 282.Google Scholar
Mangino, M. E. & Brunner, J. R. (1975). Journal of Dairy Science 58, 313.Google Scholar
Martel, M. B., Dubois, P. & Got, R. (1973). Biochimica et Biophysica Acta 311, 565.Google Scholar
Mather, I. H. & Keenan, T. W. (1974). FEBS Letters 44, 79.CrossRefGoogle Scholar
Mather, I. H. & Keenan, T. W. (1975). Journal of Membrane Biology 21, 65.Google Scholar
Moore, S. (1972). In Chemistry and Biology of Peptides, p. 629. (Ed. Meienhofer, J..) Michigan: Ann Arbor Pub.Google Scholar
Moss, B. & Rosenblum, E. N. (1972). Journal of Biological Chemistry 247, 5194.Google Scholar
Rosenberg, S. A. & Guidotti, G. (1969). Journal of Biological Chemistry 244, 5118.Google Scholar
Swope, F. C., Rhee, K. C. & Brunner, J. R. (1968). Milchwissenschaft 23, 744.Google Scholar
Wooding, F. B. P. (1971 a). Journal of Cell Science 9, 805.Google Scholar
Wooding, F. B. P. (1971 b). Journal of Ultrastructure Research 37, 388.Google Scholar