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The functions and mechanisms of sequence differences of DGAT1 gene on milk fat synthesis between dairy cow and buffalo

Published online by Cambridge University Press:  02 June 2020

Dinesh Bhattarai ,
Rahim Dad ,
Tesfay Worku ,
Sutong Xu ,
Farman Ullah ,
Min Zhang ,
Xianwei Liang ,
Tingxian Den ,
Mingxia Fan  and
Shujun Zhang
Dinesh Bhattarai*
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan430070, People's Republic of China
Rahim Dad
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan430070, People's Republic of China
Tesfay Worku
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan430070, People's Republic of China
Sutong Xu
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan430070, People's Republic of China
Farman Ullah
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan430070, People's Republic of China
Min Zhang
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan430070, People's Republic of China
Xianwei Liang
Affiliation:
Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning53000, People's Republic of China
Tingxian Den
Affiliation:
Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning53000, People's Republic of China
Mingxia Fan
Affiliation:
Renmin Hospital of Wuhan University, Wuhan430060, People's Republic of China
Shujun Zhang
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan430070, People's Republic of China
*
Author for correspondence: Dinesh Bhattarai, Email: dbhattarai@webmail.hzau.edu.cn
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Abstract

In this research communication we describe the DGAT1 sequence and promoter region in dairy cows and buffalo and compare the activities of DGAT1 between the two species in order to increase knowledge of the cause of milk fat variation. pGL-3 basic vectors were used to construct the reporter gene. Based on the predicted promoter region, 4 truncated plasmid vectors were constructed in cow-DGAT1 and 3 plasmid vectors in buffalo-DGAT1. Each reporter plasmid was transfected into the bovine mammary epithelial cell (BMEC), 293T cell, and CHO cells to analyze the activity using Dual-Luciferase Reporter Assay System. The results show that the region between −93 to −556 bp was essential for cow promoter activity while −84 to −590 bp was essential for buffalo promoter activity revealing these regions contain core promoter. The buffalo has higher promoter activity than cow yet it was not statistically significant. Comparison of candidate mutation K232A between cow and buffalo population revealed the presence of both the allelic population in dairy cows (lysine and alanine) however, only K (lysine) allelic amino acid was found in buffalo population. The absence of the alanine allelic population from buffalo explains the higher fat content of buffalo milk.

Keywords

DGAT1K232Amilk fatplasmid vectorspromoter analysis
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 2 , May 2020 , pp. 170 - 174
Copyright
Copyright © Hannah Dairy Research Foundation 2020

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References

Argov-Argaman, N, Mida, K, Cohen, BC, Visker, M and Hettinga, K (2013) Milk fat content and DGAT1 genotype determine lipid composition of the milk fat globule membrane. PLoS ONE 8, e68707.CrossRefGoogle ScholarPubMed
Bhattarai, D, Chen, X, Rehman, Z, Hao, X, Ullah, F, Dad, R, Talpur, H, Kadariya, I, Cui, L, Fan, M and Zhang, S (2017) Association of MAP4K4 gene single nucleotide polymorphism with mastitis and milk traits in Chinese Holstein cattle. Journal of Dairy Research 84, 76–79.CrossRefGoogle ScholarPubMed
Bhattarai, D, Worku, T, Dad, R, Rehman, ZU, Gong, X and Zhang, S (2018) Mechanism of pattern recognition receptors (PRRs) and host pathogen interplay in bovine mastitis. Microbial Pathogenesis 120, 64–70.CrossRefGoogle ScholarPubMed
Bovenhuis, H, Visker, M, Poulsen, NA, Sehested, J, van Valenberg, H, van Arendonk, J, Larsen, LB and Buitenhuis, AJ (2016) Effects of the diacylglycerol o-acyltransferase 1 (DGAT1) K232A polymorphism on fatty acid, protein, and mineral composition of dairy cattle milk. Journal of Dairy Science 99, 3113–3123.CrossRefGoogle ScholarPubMed
Cardoso, DF, de Souza, GF, Aspilcueta-Borquis, RR, Neto, FA, de Camargo, GM, Hurtado-Lugo, NA, Scalez, DC, de Freitas, AC, Albuquerque, LG and Tonhati, H (2015) Variable number of tandem repeat polymorphisms in DGAT1 gene of buffaloes (Bubalus bubalis) is associated with milk constituents. Journal of Dairy Science 93, 492–495.Google Scholar
Cases, S, Smith, SJ, Zheng, YW, Myers, HM, Lear, SR, Sande, E, Novak, S, Collins, C, Welch, CB, Lusis, AJ, Erickson, SK and Farese, RV (1998) Identification of a gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. Proceedings of the National Academy of Sciences of the United States of America 95, 13018–13023.CrossRefGoogle ScholarPubMed
Freitas, AC, de Camargo, GM, Aspilcueta-Borquis, RR, Stafuzza, NB, Venturini, GC, Tanamati, F, Hurtado-Lugo, NA, Barros, CC and Tonhati, H (2016 a) Polymorphism in the A2M gene associated with high-quality milk in Murrah buffaloes (Bubalus bubalis). Genetics and Molecular Research: Genetics Molecular Research 15, 1–7.CrossRefGoogle Scholar
Freitas, AC, de Camargo, GM, Stafuzza, NB, Aspilcueta-Borquis, RR, Venturini, GC, Dias, MM, Cardoso, DF and Tonhati, H (2016 b) Genetic association between SNPs in the DGAT1 gene and milk production traits in Murrah buffaloes. Tropical Animal Health and Production 48, 1421–1426.10.1007/s11250-016-1110-xCrossRefGoogle ScholarPubMed
Gautier, M, Capitan, A, Fritz, S, Eggen, A, Boichard, D and Druet, T (2007) Characterization of the DGAT1 K232A and variable number of tandem repeat polymorphisms in French dairy cattle. Journal of Dairy Science 90, 2980–2988.CrossRefGoogle ScholarPubMed
Li, S, Wang, L, He, X, Xie, Y and Zhang, Z (2015) Identification and analysis of the promoter region of the STGC3 gene. Archives of Medical Science 11, 1095–1100.Google ScholarPubMed
Ménard, O, Ahmad, S, Rousseau, F, Briard-Bion, V, Gaucheron, F and Lopez, C (2010) Buffalo vs. cow milk fat globules: size distribution, zeta-potential, compositions in total fatty acids and in polar lipids from the milk fat globule membrane. Food Chemistry 120, 544–551.10.1016/j.foodchem.2009.10.053CrossRefGoogle Scholar
Parikh, RC, Patel, JV, Patel, AB, Patel, KS, Patel, TB, Patil, RC, Kansara, JD, Jakhesara, SJ and Rank, DN (2016) DGAT1 Gene polymorphisms and its association with milk production traits in Mehsana buffalo (Bubalus bubalis). Buffalo Bulletin 2, 237–246.Google Scholar
Sandelin, A, Carninci, P, Lenhard, B, Ponjavic, J, Hayashizaki, Y and Hume, DA (2007) Mammalian RNA polymerase II core promoters: insights from genome-wide studies. Nature Reviews Genetics 8, 424–436.CrossRefGoogle ScholarPubMed
Silva, CS, Silva Filho, E, Matos, AS, Schierholt, AS, Costa, MR, Marques, LC, Costa, JS, Sales, RL, Figueiró, MR and Marques, JR (2016) Polymorphisms in the DGAT1 gene in buffaloes (Bubalus bubalis) in the Amazon. Genetics and Molecular Research 15, 3.CrossRefGoogle ScholarPubMed
White, SL, Bertrand, JA, Wade, MR, Washburn, SP, Green, JT and Jenkins, TC (2001) Comparison of fatty acid content of milk from Jersey and Holstein cows consuming pasture or a total mixed ration. Journal of Dairy Science 84, 2295–2301.CrossRefGoogle ScholarPubMed
Winter, A, Krämer, W, Werner, FA, Kollers, S, Kata, S, Durstewitz, G, Buitkamp, J, Womack, JE, Thaller, G and Fries, R (2002) Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA: diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. Proceedings of the National Academy of Sciences 9, 9300–9305.CrossRefGoogle Scholar
Yuan, J, Zhou, J, Deng, X, Hu, X and Li, N (2007) Molecular cloning and single nucleotide polymorphism detection of buffalo DGAT1 gene. Biochemical Genetics 45, 611–621.CrossRefGoogle ScholarPubMed
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