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Extraction of the 260 nm-absorbing material from Group N streptococci as a method for estimating cell growth

Published online by Cambridge University Press:  01 June 2009

D. McC. Hogg  and
G. R. Jago
D. McC. Hogg
Affiliation:
Russell Grimwade School of Biochemistry, University of Melbourne and Division of Dairy Research, C.S.I.R.O., Melbourne, Australia
G. R. Jago
Affiliation:
Russell Grimwade School of Biochemistry, University of Melbourne and Division of Dairy Research, C.S.I.R.O., Melbourne, Australia
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Summary

A method is described for estimating the growth of Group N streptococci in milk and other media by measuring the 260 nm-absorbing material extracted from the cells with 0·1 M-NaOH at 100°C. A good correlation between the absorbance at 260 nm of the hot alkali extracts and the dry weight of the cells was obtained for cultures of Streptococcus lactis during the logarithmic and early stationary phases of growth.

Type
Original Articles
Information
Journal of Dairy Research , Volume 37 , Issue 2 , June 1970 , pp. 199 - 202
Copyright
Copyright © Proprietors of Journal of Dairy Research 1970

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References

REFERENCES

Jago, G. R. (1957). Ph.D. thesis, University of Melbourne.Google Scholar
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Mitchell, P. & Moyle, J. (1951). J. gen. Microbiol. 5, 981.CrossRefGoogle Scholar