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Extraction of plasma triacylglycerols by the mammary gland of the lactating cow

Published online by Cambridge University Press:  01 June 2009

Gordon E. Thompson  and
William W. Christie
Gordon E. Thompson
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
William W. Christie
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
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Summary

The fatty acid content of triacylglycerols (TG) in arterial and mammary venous blood plasma has been analysed in five cows that were close to peak lactation. The fatty acids in arterial TG were extracted by the mammary gland in amounts 14:0 < 16:0 < 18:0and 18:0 > 18:1n – 9 > 18:2. The difference of extraction between palmitate and stearate was similar in different TG species. The fatty acid 18:1n – 7, which was largely the trans isomer, was extracted more than 18:1n – 9. Significant amounts of phytanic acid were acylated in plasma TG, but not extracted by the mammary gland.

Type
Original Articles
Information
Journal of Dairy Research , Volume 58 , Issue 3 , August 1991 , pp. 251 - 255
Copyright
Copyright © Proprietors of Journal of Dairy Research 1991

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References

REFERENCES

Annison, E. F., Linzell, J. L., Fazakerley, S. & Nichols, B. W. 1967 The oxidation and utilization of palmitate, stearate, oleate and acetate by the mammary gland of the fed goat in relation to their overall metabolism and the role of plasma phospholipids and neutral lipids in milk-fat synthesis. Biochemical Journal 102 637–647CrossRefGoogle ScholarPubMed
Christie, W. W. 1989 HPLC and GC-mass spectrometry in the analysis of fatty acids. In Fats for the Future pp. 335–344 (Ed. Cambie, R. C.) Chichester: Ellis Horwood LtdGoogle Scholar
Christie, W. W. 1990 Silver ion chromatography of triacylglycerols on solid phase extraction columns packed with a bonded sulphonic acid phase. Journal of the Science of Food and Agriculture 52 573–577CrossRefGoogle Scholar
Christie, W. W., Clegg, R. A., Calvert, D. T. & Noble, R. C. 1984 The positional distributions of fatty acids in the triacylglycerols and phosphatidylcholines of the intestinal and popliteal lymph and plasma of sheep. Lipids 19 982–986CrossRefGoogle ScholarPubMed
Christie, W. W. & Moore, J. H. 1971 Structures of triglycerides isolated from various sheep tissues. Journal of the Science of Food and Agriculture 22 120–124CrossRefGoogle Scholar
Glascock, R. F. & Welch, V. A. 1974 Contribution of the fatty acids of three low density serum lipoproteins to bovine milk fat. Journal of Dairy Science 57 1364–1370CrossRefGoogle ScholarPubMed
Gleeson, M. & Maugham, R. J. 1986 A simple enzymatic fluorimetric method for the determination of triglycerides in 10 μl of serum. Clinica Chimica Acta 156 97–104.CrossRefGoogle Scholar
Moore, J. H. & Christie, W. W. 1979 Lipid metabolism in the mammary gland of ruminant animals. Progress in Lipid Research 17 347–395CrossRefGoogle ScholarPubMed
Olivecrona, T. & Bengtsson-Olivecrona, G. 1987 Lipoprotein lipase from milk – the model enzyme in lipoprotein lipase research. In Lipoprotein Lipase pp. 15–58 (Ed. Borensztajn, J.) Chicago: Evener Publishers Inc.Google ScholarPubMed
Snedecor, G. W. & Cochran, W. G. 1956 Statistical Methods, 5th edn.Ames, IA: Iowa State University PressGoogle Scholar
Zottor, B. A. K. & Walker, B. L. 1989 Relative hydrolysis of trans- and cis-triglyceride by rat mammary lipoprotein lipase. Nutrition Research 9 679–683CrossRefGoogle Scholar