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Examination of methionine stimulation of gene expression in dairy cow mammary epithelial cells using RNA-sequencing

Published online by Cambridge University Press:  07 May 2020

Xiaoming Hou ,
Minghui Jiang ,
Jinyu Zhou ,
Shuyuan Song ,
Feng Zhao  and
Ye Lin
Xiaoming Hou
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin150030, China
Minghui Jiang
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin150030, China
Jinyu Zhou
Affiliation:
Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin150030, China
Shuyuan Song
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin150030, China
Feng Zhao
Affiliation:
Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin150030, China
Ye Lin*
Affiliation:
Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin150030, China
*
Author for correspondence: Ye Lin, Email: linlu516@163.com
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Abstract

In this research communication, a cell model with elevated β-CASEIN synthesis was established by stimulating bovine mammary epithelial cells with 0.6 mM methionine, and the genome-wide gene expression profiles of methionine-stimulated cells and untreated cells were investigated by RNA sequencing. A total of 458 differentially expressed genes (DEGs; 219 upregulated and 239 downregulated) were identified between the two groups. Gene Ontology (GO) analysis showed that the two highest-ranked GO terms in ‘molecular function’ category were ‘binding’ and ‘catalytic activity’, suggesting that milk protein synthesis in methionine-stimulated cells requires induction of gene expression to increase metabolic activity. Kyoto Encyclopedia of Genes and Genomes analysis revealed that within the ‘environmental information processing’ category, the subcategory that is most highly enriched for DEGs was ‘signal transduction’. cGMP-PKG, Rap1, calcium, cAMP, PI3K-AKT, MAPK, and JAK-STAT are the pathways with the highest number of DEGs, suggesting that these signaling pathways have potential roles in mediating methionine-induced milk protein synthesis in bovine mammary epithelial cells. This study provides valuable insights into the physiological and metabolic adaptations in cells stimulated with methionine. Understanding the regulation of this transition is essential for effective intervention in the lactation process.

Keywords

Bovine mammary epithelial cellmethioninemilk protein synthesisRNA sequencing
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 2 , May 2020 , pp. 226 - 231
Copyright
© The Author(s), 2020. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation.

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