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Enumeration of starter cultures in fermented milks

Published online by Cambridge University Press:  01 June 2009

Hamid B. Ghoddusi  and
Richard K. Robinson
Hamid B. Ghoddusi
Affiliation:
Department of Food Science and Technology, University of Reading, Whiteknights, Reading RG6 2AP, UK
Richard K. Robinson
Affiliation:
Department of Food Science and Technology, University of Reading, Whiteknights, Reading RG6 2AP, UK
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Summary

Some media available for the isolation and enumeration of starter cultures employed for the manufacture of cheese, yogurt and bio-yogurt were examined. Reddy's medium or a modification of Elliker's medium was found to be most satisfactory for Lactococcus spp., while trypticase phytone yeast (TPY) agar with a mixture of antibiotics proved suitable for the discrete enumeration of Bifidobacterium spp. The inclusion of Prussian blue (PB) in reinforced clostridial medium or tryptone proteose peptone yeast extract (TPPY) agar gave excellent differential counts for the starter bacteria in yogurt even when the culture was imbalanced, while TPPY (PB) agar allowed the visible separation of all four of the organisms that might be found in a typical bio-yogurt, namely Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus thermophilus, a, Bifidobacterium sp. and Lb. acidophilus. It was noted that variation among different strains of any given species could change the expected reactions, so for quality control purposes the suggested media may need to be modified to cope with the specific cultures in use.

Type
Original Articles
Information
Journal of Dairy Research , Volume 63 , Issue 1 , February 1996 , pp. 151 - 158
Copyright
Copyright © Proprietors of Journal of Dairy Research 1996

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