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Elevation of bovine serum C-reactive protein and serum amyloid P component levels by lactation

Published online by Cambridge University Press:  01 June 2009

Masami Morimatsu ,
Atsushi Watanabe ,
Kumiko Yoshimatsu ,
Toru Fujinaga ,
Masahiko Okubo  and
Masaharu Naiki
Masami Morimatsu
Affiliation:
Department of Biochemistry, Hokkaido University, Sapporo 060, Japan
Atsushi Watanabe
Affiliation:
Department of Biochemistry, Hokkaido University, Sapporo 060, Japan
Kumiko Yoshimatsu
Affiliation:
Department of Biochemistry, Hokkaido University, Sapporo 060, Japan
Toru Fujinaga
Affiliation:
Department of Surgery, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060, Japan
Masahiko Okubo
Affiliation:
Department of Animal Science, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan
Masaharu Naiki
Affiliation:
Department of Veterinary Science, National Institute of Health, Tokyo 141, Japan
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Summary

C-reactive protein (CRP) and serum amyloid P component (SAP), which are known to increase in sera from humans and many other animals with acute inflammation caused by infection, toxic drug administration or injury, were previously purified from bovine serum. These serum levels were determined by enzyme-linked immunosorbent assay (ELISA) using specific antiserum to bovine CRP or SAP which was prepared by immunizing rabbits and goats with each purified protein. Among 68 healthy Holstein cows, 45 non-lactating cows had levels of CRP and SAP of 20·6±1·4 and 27·6 ± 1·3 μg/ml respectively; 23 lactating cows had higher levels of CRP and SAP (76·0±13·6 and 38·3±5·5 μg/ml respectively). In the latter group, there was a high correlation between milk yield and serum CRP levels (P <0·001). From these observations, it was assumed that lactation might stimulate CRP synthesis rather than SAP synthesis in bovine liver as an acute phase reaction, and that CRP might be called a lactation-associated protein.

Type
Original Articles
Information
Journal of Dairy Research , Volume 58 , Issue 3 , August 1991 , pp. 257 - 261
Copyright
Copyright © Proprietors of Journal of Dairy Research 1991

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References

REFERENCES

Bendtzen, K. 1988 Interleukin 1, interleukin 6 and tumor necrosis factor in infection, inflammation and immunity. Immunology Letters 19 183–192.CrossRefGoogle ScholarPubMed
Forsyth, I. A. 1983 The endocrinology of lactation. In Biochemistry of Lactation pp. 309–349 (Ed. Mepham, T. B.) Amsterdam: ElsevierGoogle Scholar
Kindmark, C.-O. 1972 The concentration of C-reactive protein in sera from healthy individuals. Scandinavian Journal of Clinical Investigation 29 407–411.CrossRefGoogle ScholarPubMed
Maudsley, S., Rowe, I. F., De Beer, F. C., Munn, E. A., Herbert, J., Feinstein, A. & Pepys, M. B. 1987 Identification and isolation of two pentaxins from bovine serum. Clinical and Experimental immunology 67 662–673.Google Scholar
Morimatsu, M., Sakai, H., Yoshimatsu, K., Minowa, O., Yamamoto, S., Yatomi, K., Fujinaga, T. & Naiki, M. 1989 Isolation and characterization of C-reactive protein and serum amyloid P component from bovine serum. Japanese Journal of Veterinary Science 51 723–732Google ScholarPubMed
Pepys, M. B. & Baltz, M. L. 1983 Acute-phase proteins with special reference to C-reactive protein and related proteins (pentaxins) and serum amyloid A protein. Advances in Immunology 34 141–212.CrossRefGoogle ScholarPubMed
Pepys, M. B., Baltz, M. L., Gomer, K., Davies, A. J. S. & Doenhoff, M. 1979 Serum amyloid P-component is an acute-phase reactant in the mouse. Nature 278 259–261CrossRefGoogle ScholarPubMed
Romem, Y. & Artal, R. 1985 C-reactive protein in pregnancy and in the postpartum period. American Journal of Obstetrics and Gynecology 151 380–383.CrossRefGoogle ScholarPubMed
Rozansky, R. & Bercovici, B. 1956 C-reactive protein during pregnancy and in cord blood. Proceedings of the Society for Experimental Biology and Medicine 92 4–6CrossRefGoogle ScholarPubMed
Salonen, E.-M. 1982 A rapid and sensitive solid-phase enzyme immunoassay for C-reactive protein. Journal of Immunological Methods 48 45–50CrossRefGoogle ScholarPubMed
Shephard, E. G., van Helden, P. D., Strauss, M., Bohm, L. & De Beer, F. C. 1986 Functional effects of CRP binding to nuclei. Immunology 58 489–494Google ScholarPubMed