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Effect of experimental stress and cortisol release induced by ACTH administration on expression of key genes related to milk synthesis and apoptosis during mammary involution of Saanen goats

Published online by Cambridge University Press:  18 November 2022

Emanuel Manica ,
Priscila dos Santos Silva ,
Giovana Krempel Fonseca Merighe ,
Sandra Aparecida de Oliveira ,
Gabriela Facholi Bomfim  and
João Alberto Negrão
Emanuel Manica
Affiliation:
Department of Basic Sciences, Faculty of Animal Science and Food Engineering, University of Sao Paulo, Pirassununga, SP 05508-270, Brazil
Priscila dos Santos Silva
Affiliation:
Department of Basic Sciences, Faculty of Animal Science and Food Engineering, University of Sao Paulo, Pirassununga, SP 05508-270, Brazil
Giovana Krempel Fonseca Merighe
Affiliation:
Department of Basic Sciences, Faculty of Animal Science and Food Engineering, University of Sao Paulo, Pirassununga, SP 05508-270, Brazil
Sandra Aparecida de Oliveira
Affiliation:
Department of Basic Sciences, Faculty of Animal Science and Food Engineering, University of Sao Paulo, Pirassununga, SP 05508-270, Brazil
Gabriela Facholi Bomfim
Affiliation:
Department of Basic Sciences, Faculty of Animal Science and Food Engineering, University of Sao Paulo, Pirassununga, SP 05508-270, Brazil
João Alberto Negrão*
Affiliation:
Department of Basic Sciences, Faculty of Animal Science and Food Engineering, University of Sao Paulo, Pirassununga, SP 05508-270, Brazil
*
Author for correspondence: João A. Negrão, Email: jnegrao@usp.br
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Abstract

This research paper addresses the hypothesis that stress, induced by ACTH administration and cortisol release increases somatic cell count (SCC) in mammary secretion, and improves the effectiveness of dry off in goats. We report indicators of milk synthesis and mammary gland involution during dry off. Thirty Saanen goats were subjected to abrupt dry off and treatments: (1) ACTH administration (ACTH) or (2) placebo (Control) on days 1, 3, 6, 9, 12, 15, 30, and 60 of dry off. The expression of target genes in mammary tissue that are related to milk synthesis and cell survival such as insulin-like growth factor 1 receptor (IGF1R), phosphatidylinositol-3-kinase (PIK3CA), protein kinase B (AKT1) and mechanistic target of rapamycin (MTOR), casein (CSN2), lactalbumin (LALBA) and lactoferrin (LF) were evaluated, and plasma cortisol concentration, SCC, leucocyte count, and microbiological analyses in milk and mammary secretions were assessed. ACTH significantly downregulated the expression of IGF1R and upregulated the expression of PIK3CA in mammary tissue, increased lactoferrin concentration and SCC, and changed immune cell levels in mammary secretions compared to Control. Furthermore, ACTH administration increased the percentage of dry goats compared to the Control (73 vs. 46%, respectively). We conclude that the effect of stress via ACTH administration and cortisol release accelerated mammary involution during the early dry-off period.

Keywords

Cell survivaldairy goatsdry off periodglucocorticoid
Type
Research Article
Information
Journal of Dairy Research , Volume 89 , Issue 4 , November 2022 , pp. 404 - 409
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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