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Dominance of the genus Polaromonas in the microbial ecology of an Intermittently Aerated Sequencing Batch Reactor (IASBR) treating dairy processing wastewater under varying aeration rates

Published online by Cambridge University Press:  08 August 2018

Beatriz Gil-Pulido ,
Emma Tarpey ,
William Finnegan ,
Xinmin Zhan ,
Alan DW Dobson  and
Niall O'Leary
Beatriz Gil-Pulido
Affiliation:
School of Microbiology, University College Cork, College Road, Cork T12 YN60, Ireland Environmental Research Institute, University College Cork, Lee Road, Cork T23 XE10, Ireland
Emma Tarpey
Affiliation:
College of Engineering and Informatics, National University of Ireland Galway, University Road, Galway H91 TK33, Ireland
William Finnegan
Affiliation:
College of Engineering and Informatics, National University of Ireland Galway, University Road, Galway H91 TK33, Ireland
Xinmin Zhan
Affiliation:
College of Engineering and Informatics, National University of Ireland Galway, University Road, Galway H91 TK33, Ireland
Alan DW Dobson
Affiliation:
School of Microbiology, University College Cork, College Road, Cork T12 YN60, Ireland Environmental Research Institute, University College Cork, Lee Road, Cork T23 XE10, Ireland
Niall O'Leary*
Affiliation:
School of Microbiology, University College Cork, College Road, Cork T12 YN60, Ireland Environmental Research Institute, University College Cork, Lee Road, Cork T23 XE10, Ireland
*
*For correspondence; e-mail: n.oleary@ucc.ie
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Abstract

In this Research Communication we investigate potential correlations between key bacterial groups and nutrient removal efficiency in an Intermittently Aerated Sequencing Batch Reactor (IASBR) treating synthetic dairy processing wastewater. Reactor aeration rates of 0·6 and 0·4 litre per minute (LPM) were applied to an 8 l laboratory scale system and the relative impacts on IASBR microbial community structure and orthophosphate (PO4-P) and ammonium (NH4-N) removal efficiencies compared. Aeration at 0·6 LPM over several sludge retention times (SRTs) resulted in approximately 92% removal efficiencies for both PO4-P and NH4-N. Biomass samples subjected to next-generation sequencing (NGS), 16S rRNA profiling revealed a concomitant enrichment of Polaromonas under 0·6 LPM conditions, up to ~50% relative abundance within the reactor biomass. The subsequent shift in reactor aeration to 0·4 LPM, over a period of 3 SRTs, resulted in markedly reduced nutrient removal efficiencies for PO4-P (50%) and NH4-N (45%). An 85·7% reduction in the genus level relative abundance of Polaromonas was observed under 0·4 LPM aeration conditions over the same period.

Keywords

Dairy wastewaterbioremediationnutrient removal
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 3 , August 2018 , pp. 388 - 390
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

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