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Characterization and response to mitogens of mammary lymphocytes from the bovine dry-period secretion

Published online by Cambridge University Press:  01 June 2009

Carlos Concha ,
Olof Holmberg  and
Bror Morein
Carlos Concha
Affiliation:
Department of Biological Products, National Veterinary Institute, S-104 05 Stockholm, Sweden
Olof Holmberg
Affiliation:
Department of Bacteriology, National Veterinary Institute, S-750 07 Uppsala, Sweden
Bror Morein
Affiliation:
Department of Biological Products, National Veterinary Institute, S-104 05 Stockholm, Sweden
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Summary

From bovine mammary secretion during the dry period, the total number of cells was between 1·2 and 5·9 Ǻ 106/ml. A mean of 35% of these cells were classified as lymphocytes and ∼ 85% of them could be isolated by the Ficoll-isopac method. Centrifugation separated 6% of the cells into the fat; 5% of them were lymphocytes. About 47% of the lymphocytes bound Helix pomatia agglutinin, a T-cells marker, while the proportion of Ig-bearing cells was ∼ 28%. The mammary lymphocytes were stimulated by the lectins phytohaemagglutinin, pokeweed mitogen, concanavalin A and by lipopolysaccharide from Salmonella typhimurium. The stimulation indices of mammary lymphocytes were generally lower than those for peripheral blood lymphocytes from the same animals. The background values, i.e. counts/min of lymphocytes incubated without mitogen, were often higher for lymphocytes isolated from mammary secretion than from blood.

Type
Original Articles
Information
Journal of Dairy Research , Volume 47 , Issue 3 , October 1980 , pp. 305 - 311
Copyright
Copyright © Proprietors of Journal of Dairy Research 1980

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