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Bovine milk esterases

Published online by Cambridge University Press:  01 June 2009

B. J. Kitchen
B. J. Kitchen
Affiliation:
Otto Madsen Dairy Research Laboratory, Harbour Road, Hamilton 4007, Brisbane, Australia
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Summary

The type and distribution of esterases in milk has been investigated using selective inhibitors during normal assay procedures and during histochemical staining of polyacrylamide gels. Enzyme solutions were obtained from skim-milk by acid and alkali precipitation, followed by ammonium sulphate fractionation, ultra-filtration and Sephadex G-100 chromatography. The major type of esterase present was an aryl-esterase (E.C. 5.1.1.2) while a smaller amount of a choline-ester hydrolase (E.C. 3.1.1.7; 3.1.1.8) was detected. The significance of these findings is discussed.

Type
Original Articles
Information
Journal of Dairy Research , Volume 38 , Issue 2 , June 1971 , pp. 171 - 177
Copyright
Copyright © Proprietors of Journal of Dairy Research 1971

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