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Association of SNP and STR polymorphisms of insulin-like growth factor 2 receptor (IGF2R) gene with milk traits in Holstein-Friesian cows

Published online by Cambridge University Press:  22 May 2018

Marta Dux ,
Magdalena Muranowicz ,
Eulalia Siadkowska ,
Dagmara Robakowska-Hyżorek ,
Krzysztof Flisikowski ,
Emilia Bagnicka  and
Lech Zwierzchowski
Marta Dux
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, 05-552 Jastrzębiec, Postępu 36a, Poland
Magdalena Muranowicz
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, 05-552 Jastrzębiec, Postępu 36a, Poland
Eulalia Siadkowska
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, 05-552 Jastrzębiec, Postępu 36a, Poland
Dagmara Robakowska-Hyżorek
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, 05-552 Jastrzębiec, Postępu 36a, Poland
Krzysztof Flisikowski
Affiliation:
Chair of Livestock Biotechnology, Technical University of Munich, Liesel-Beckmannstr.1, D-85354 Freising, Germany
Emilia Bagnicka
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, 05-552 Jastrzębiec, Postępu 36a, Poland
Lech Zwierzchowski*
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, 05-552 Jastrzębiec, Postępu 36a, Poland
*
*For correspondence; e-mail: l.zwierzchowski@ighz.pl
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Abstract

The objective of the study reported in this Research Communication was to investigate the association of polymorphisms in the insulin-like growth factor receptor 2 (IGF2R) gene with milk traits in 283 Polish Holstein-Friesian (PHF) cows from the IGAB PAS farm in Jastrzębiec. IGF2R regulates the availability of biologically active IGF2 which is considered as a genetic marker for milk or meat production in farm animals. Two novel genetic polymorphisms were identified in the bovine IGF2R gene: a polymorphic TG-repeat in intron 23 (g.72389 (TG)15-67), and a g.72479 G > A SNP RFLP-StyI in exon 24. The following milk traits were investigated: milk yield, protein and fat yield, SCC and lactose content. To determine the influence of the IGF2R STR and SNP genotypes on the milk traits, we used the AI-REML (average information restricted maximum likelihood) method with repeatability, multi-trait animal model based on test-day information using DMU package. Statistical analysis revealed that the G/A genotype (P ≤ 0·01) was associated with milk and protein yield, lactose content and somatic cell count (SCC) in Polish HF cows. TGn (29/22, 28/29, 28/22, 28/28) genotypes were associated with high values for milk, (28/22, 28/23) with protein and fat yield, (25/20) with lactose content, and (29/33, 28/28) with low SCC. We suggest that the IGF2R gene polymorphisms could be useful genetic markers for dairy production traits in cattle.

Keywords

TGn microsatelliteA/G transitiongenetic markerassociationdairy traits
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 2 , May 2018 , pp. 138 - 141
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

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