Hostname: page-component-76fb5796d-qxdb6 Total loading time: 0 Render date: 2024-04-26T03:45:05.362Z Has data issue: false hasContentIssue false
  • English
  • Français

Application of a linear regression model to study the origin of C17 branched-chain fatty acids in caprine milk fat

Published online by Cambridge University Press:  14 November 2019

Pilar Gómez-Cortés ,
Alfonso Cívico ,
Miguel Angel de la Fuente  and
Andrés L. Martínez Marín
Pilar Gómez-Cortés
Affiliation:
Instituto de Investigación en Ciencias de la Alimentación (CSIC-UAM), Universidad Autónoma de Madrid, Nicolás Cabrera 9, 28049Madrid, Spain
Alfonso Cívico
Affiliation:
Departamento de Producción Animal, Universidad de Córdoba, Ctra. Madrid-Cádiz km 396, 14071Córdoba, Spain
Miguel Angel de la Fuente
Affiliation:
Instituto de Investigación en Ciencias de la Alimentación (CSIC-UAM), Universidad Autónoma de Madrid, Nicolás Cabrera 9, 28049Madrid, Spain
Andrés L. Martínez Marín*
Affiliation:
Departamento de Producción Animal, Universidad de Córdoba, Ctra. Madrid-Cádiz km 396, 14071Córdoba, Spain
*
Author for correspondence: Andrés L. Martínez Marín, Email: pa1martm@uco.es
Get access Rights & Permissions [Opens in a new window]

Abstract

This research communications addresses the hypothesis that a part of iso 17:0 and anteiso 17:0 in milk fat could come from endogenous extraruminal tissue synthesis. In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively. Sixteen dairy goats were allocated to two simultaneous experiments, in a crossover design with four animals per treatment and two experimental periods of 25 d. In both experiments, alfalfa hay was the sole forage and the forage to concentrate ratio (33 : 67) remained constant. Experimental diets differed on the concentrate composition, either rich in starch or neutral detergent fibre, and they were administered alone or in combination with 30 g/d of linseed oil. Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns. The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.

Keywords

Branched-chain fatty acidelongasegoatmilk
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 4 , November 2019 , pp. 461 - 463
Copyright
Copyright © Hannah Dairy Research Foundation 2019

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bichi, E, Toral, PG, Hervás, G, Frutos, P, Gómez-Cortés, P, Juárez, M and De la Fuente, MA (2012) Inhibition of Δ9-desaturase activity with sterculic acid: effect on the endogenous synthesis of cis-9 18:1 and cis-9 trans-11 18:2 in dairy sheep. Journal of Dairy Science 95, 5242–5252.CrossRefGoogle Scholar
Castro-Carrera, T, Frutos, P, Leroux, C, Chilliard, Y, Hervás, G, Belenguer, A, Bernard, L and Toral, PG (2015) Dietary sunflower oil modulates milk fatty acid composition without major changes in adipose and mammary tissue fatty acid profile or related gene mRNA abundance in sheep. Animal: An International Journal of Animal Bioscience 9, 582–591.10.1017/S1751731114002882CrossRefGoogle ScholarPubMed
Cívico, A, Núñez Sánchez, N, Gómez-Cortés, P, De la Fuente, MA, Peña Blanco, F, Juárez, M, Schiavone, A and Martínez Marín, AL (2017) Odd- and branched-chain fatty acids in goat milk as indicators of the diet composition. Italian Journal of Animal Science 16, 68–74.CrossRefGoogle Scholar
Craninx, M, Steen, A, Van Laar, H, Van Nespen, T, Martin-Tereso, J, De Baets, B and Fievez, V (2008) Effect of lactation stage on the odd-and branched-chain milk fatty acids of dairy cattle under grazing and indoor conditions. Journal of Dairy Science 91, 2662–2677.CrossRefGoogle ScholarPubMed
European Commission (2010) Directive 2010/63/EU of the European Parliament and of 58 the Council of 22 September 2010 on the protection of animals used for scientific purposes. Official Journal of European Union 276, 33–79.Google Scholar
Fievez, V, Colman, E, Castro-Montoya, JM, Stefanov, I and Vlaeminck, B (2012) Milk odd-and branched-chain fatty acids as biomarkers of rumen function—an update. Animal Feed Science and Technology 172, 51–65.CrossRefGoogle Scholar
French, EA, Bertics, SJ and Armentano, LE (2012) Rumen and milk odd-and branched-chain fatty acid proportions are minimally influenced by ruminal volatile fatty acid infusions. Journal of Dairy Science 95, 2015–2026.CrossRefGoogle ScholarPubMed
Palmquist, DL, St. Pierre, N and McClure, KE (2004) Tissue fatty acid profiles can be used to quantify endogenous rumenic acid synthesis in lambs. Journal of Nutrition 134, 2407–2414.CrossRefGoogle ScholarPubMed
Shi, HB, Wu, M, Zhu, JJ, Zhang, CH, Yao, DW, Luo, J and Loor, JJ (2017) Fatty acid elongase 6 plays a role in the synthesis of long-chain fatty acids in goat mammary epithelial cells. Journal of Dairy Science 100, 4987–4995.CrossRefGoogle Scholar
Vlaeminck, B, Fievez, V, van Laar, H, Vlaeminck, B, Fievez, V, Cabrita, ARJ, Fonseca, AJM and Dewhurst, RJ (2006) Factors affecting odd- and branched-chain fatty acids in milk: A review. Animal Feed Science and Technology 131, 389–417.CrossRefGoogle Scholar
Vlaeminck, B, Gervais, R, Rahman, MM, Gadeyne, F, Gorniak, M, Doreau, M and Fievez, V (2015) Postruminal synthesis modifies the odd- and branched-chain fatty acid profile from the duodenum to milk. Journal of Dairy Science 98, 4829–4840.CrossRefGoogle Scholar
Xu, HF, Luo, J, Zhao, WS, Yang, YC, Tian, HB, Shi, HB and Bionaz, M (2016) Overexpression of SREBP1 (sterol regulatory element binding protein 1) promotes de novo fatty acid synthesis and triacylglycerol accumulation in goat mammary epithelial cells. Journal of Dairy Science 99, 783–795.CrossRefGoogle ScholarPubMed
Wang, Z, Park, HG, Wang, DH, Goykhman, Y, Lawrence, P, Kothapalli, KSD and Brenna, JT (2017) ELOVL3, 6 and 7 specifically elongate saturated branched chain fatty acids and compete with normal fatty acids. FASEB Journal 31(suppl. 1), Abs. 137.8.Google Scholar