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Analysis and production of two exopolysaccharides from Lactococcus lactis subsp. cremoris LC330

Published online by Cambridge University Press:  01 June 2009

Valerie M. Marshall ,
Eoin N. Cowie  and
Rod S. Moreton
Valerie M. Marshall
Affiliation:
School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield HD1 3DH, UK
Eoin N. Cowie
Affiliation:
Department of Biology and Molecular Sciences, Oxford Brookes University, Headington, Oxford OX3 0BP, UK
Rod S. Moreton
Affiliation:
Nestlé Research Centre, Vers-Chez-les-Blanc, CH-1000, Suisse
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Summary

Two polysaccharides produced concurrently by Lactococcus lactis subsp. cremoris strain LC330 have been identified. One had a high molecular mass (> 1 × 106 Da) and was neutral. The second was smaller (∼ 10000 Da), charged and had a high phosphorus content. Sugar composition also differed. In chemostat culture the neutral polysaccharide was influenced by temperature and by nitrogen limitation. This polysaccharide was branched with terminal galactose moieties and contained galactose, glucose and glucosamine. The phosphopolysaccharide was more complex with glucose, rhamnose, galactose and glucosamine in an approximate ratio of 6:5:4:1.

Type
Original Articles
Information
Journal of Dairy Research , Volume 62 , Issue 4 , November 1995 , pp. 621 - 628
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

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