Friesian cows averaging about 30 lb of milk daily, were used for nitrogen balance experiments of the change-over type, in which 3·0–3·5 lb of two mixtures of molasses and urea were added to basal diets low in protein, but containing a high proportion of starch and lower carbohydrates, and ample energy for the amount of milk produced. The molasses mixtures contained 9–10% urea, about 2% H3PO4 and were added either alone or with 7% ethyl alcohol.

In the first experiment, with three cows, the intake of digestible crude protein from the basal diet was about 90% of the recommended allowance and the utilization of urea nitrogen was poor. In the second experiment, with four cows, the intake of digestible crude protein with the basal diet was about 40% of the recommended allowance and the utilization of the nitrogen of urea and of additional groundnut meal was almost complete.

Owing to the way in which the experiments developed the addition of alcohol to the mixture of molasses and urea could not be expected in the present work to increase the utilization of the nitrogen of urea even if it does have that effect under other circumstances. When added to the basal diet very low in nitrogen, the nitrogen of both urea and groundnut meal was used mainly to prevent the withdrawal of nitrogen from body reserves, but partly for a small increase in milk yield.

Under the special conditions of this experiment, which were very favourable for the utilization of non-protein nitrogen, the allowances of digestible crude protein recommended by Woodman (1957) for maintenance of cows weighing 1250 lb (0·73 lb) and 1340 lb (0·82 lb) and giving about 30 lb of milk daily (0·6 lb/10 lb milk) did not appear to be excessive.