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The type of cheese curds determined the colouring capacity of Brevibacterium and Arthrobacter species

Published online by Cambridge University Press:  13 May 2010

Marie-Noëlle Leclercq-Perlat*
UMR 782 – AgroParisTech – INRA Génie et microbiologie des procédés alimentaires. BP 1 - F78850 Thiverval-Grignon
Henry-Eric Spinnler
UMR 782 – AgroParisTech – INRA Génie et microbiologie des procédés alimentaires. BP 1 - F78850 Thiverval-Grignon
*For correspondence; e-mail:


This study compares the colouring capacity of Brevibacterium aurantiacum (BA), Brevibacterium BL and Arthrobacter species AS in relation to deacidified media made from lactic curd (Epoisses), mixed curds (Munster) and rennet curds (Livarot or Reblochon). BA colouring capacity proved to be constant, leading to a dark orange colour, irrespective of the deacidified media. However, it gave too dark a colour for Reblochon. The strains BL and AS were not adapted to the colouring of Epoisses deacidified medium. On the Livarot or Munster deacidified medium, these two strains provided a light yellow orange colour range that was not suitable for these cheeses. However, these two strains (BL and AS) produced a suitable colour for Reblochon deacidified medium.

Research Article
Copyright © Proprietors of Journal of Dairy Research 2010

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Bockelmann, W, Fuehr, C, Martin, D & Heller, KJ 1997 Color development by Red-Smear surface bacteria. Kieler. Milchwirt. Forsch. 49 285292Google Scholar
Bockelmann, W, Willems, KP, Neve, H & Heller, KH 2005 Cultures for the ripening of smear cheeses. International Dairy Journal 15 719732CrossRefGoogle Scholar
Boyaval, P, Boyaval, E & Desmazeaud, MJ 1985 Survival of Brevibacterium linens during nutrient starvation and intracellular changes. Archives of Microbiology 141 128132CrossRefGoogle ScholarPubMed
Carreira, A, Dillinger, K, Eliskases-Lechner, F, Loureiro, V, Ginzinger, W & Rohm, H 2002 Influence of selected factors on browning of Camembert cheese. Journal of Dairy Research 69 281292CrossRefGoogle ScholarPubMed
Carreira, A, Paloma, L & Loureiro, V 1998 Pigment producing yeasts involved in the brown surface discoloration of ewes' cheese. International Journal of Food Microbiology 41 223230CrossRefGoogle ScholarPubMed
Corsetti, A, Rossi, J & Gobbetti, M 2001 Interactions between yeasts and bacteria in the smear surface-ripened cheeses. International Journal of Food Microbiology 69 110CrossRefGoogle ScholarPubMed
Dufossé, L, Galaup, P, Carlet, E, Flamin, C & Valla, A 2005 Spectrocolorimetry in the CIE L*a*b* color space as useful tool for monitoring the ripening process and the quality of PDO red-smear soft cheeses. Food Research International 38 919924CrossRefGoogle Scholar
Dufossé, L, Mabon, P & Binet, A 2001 Assessment of the coloring strength of Brevibacterium linens strains: Spectrocolorimetry versus total carotenoid extraction/quantification. Journal of Diary Science 84 354360CrossRefGoogle ScholarPubMed
Eliskases-Lechner, F & Ginzinger, W 1995a The bacterial flora of surface-ripened cheeses with special regard to coryneforms. Lait 75 571583CrossRefGoogle Scholar
Eliskases-Lechner, F & Ginzinger, W 1995b The yeast flora of surface-ripened cheeses. Milchwissenschaft 50 458462Google Scholar
Galaup, P, Flamin, C, Carlet, E & Dufossé, L 2005 HPLC analysis of the pigments produced by the microflora isolated from the ‘Protected Designation of Origin’ French red-smear soft cheeses Munster, Epoisses, Reblochon and Livarot. Food Research International 38 855860CrossRefGoogle Scholar
Galaup, P, Gautier, A, Piriou, Y, de Villefranche, A, Valla, A & Dufossé, L 2007 First pigment fingerprints from the rind of French PDO red-smear ripened soft cheeses Epoisses, Mont d'Or and Maroilles. Innovative Food Science and Emerging Technology 8 373378CrossRefGoogle Scholar
Guyomarc'h, F, Binet, A & Dufossé, L 2000 Characterization of Brevibacterium linens pigmentation using spectrocolorimetry. International Journal of Food Microbiology 57 201210CrossRefGoogle ScholarPubMed
Irlinger, F, Bimet, F, Delettre, J, Lefèvre, M & Grimont, PAD 2005 Arthrobacter bergerei sp. nov. and Arthrobacter arilaitensis sp. nov., novel coryneform species isolated from the surfaces of cheeses. International Journal of Systematic Evolution Microbiology 55 457462CrossRefGoogle ScholarPubMed
Krubasik, P & Sandmann, G 2000 A carotenogenic gene cluster from Brevibacterium linens with novel lycogene cyclase genes involved in the synthesis of aromatic carotenoids. Molecular General Genetic 263 423432CrossRefGoogle ScholarPubMed
Leclercq-Perlat, MN, Corrieu, G & Spinnler, HE 2004a The color of Brevibacterium linens depends on the yeast used for cheese deacidification. Journal of Dairy Science 87 15361544CrossRefGoogle ScholarPubMed
Leclercq-Perlat, MN, Corrieu, G & Spinnler, HE 2004b Comparison of Volatile Compounds Produced in Model Cheese Medium Deacidified by Debaryomyces hansenii or Kluyveromyces marxianus. Journal of Dairy Science 87 15451550CrossRefGoogle ScholarPubMed
Leclercq-Perlat, MN, Oumer, A, Bergère, JL, Spinnler, HE & Corrieu, G 1999 Growth of Debaryomyces hansenii on a bacterial surface-ripened soft cheese. Journal of Dairy Research 66 271281CrossRefGoogle Scholar
Leclercq-Perlat, MN, Oumer, A, Bergère, JL, Spinnler, HE & Corrieu, G 2000 Behavior of Brevibacterium linens and Debaryomyces hansenii as ripening flora in controlled production of soft smear cheese from reconstituted milk: growth and substrate consumption. Journal of Dairy Science 83 16651673CrossRefGoogle ScholarPubMed
Lecocq, J & Guéguen, M 1994 Effects of pH and sodium chloride on the interactions between G. candidum and Brev. linens. Journal of Dairy Science 77 28902899CrossRefGoogle Scholar
Lecocq, JGuéguen, M & Coiffier, O 1996 [The importance of the association between Geotrichum candidum and Brevibacterium linens in the ripening of cheeses with washed rind]. Sciences des Aliments 16 317327Google Scholar
Masoud, W & Jakobsen, M 2003 Surface ripened cheeses: the effects of Debaryomyces hansenii, NaCl and pH on the intensity of pigmentation produced by Brevibacterium linens and Corynebacterium flavescens. International Dairy Journal 13 231237CrossRefGoogle Scholar
Masoud, W & Jakobsen, M 2005 The combined effects of pH, NaCl and temperature on growth, of cheese ripening cultures of Debaryomyces hansenii and coryneform bacteria. International Dairy Journal 15 6977CrossRefGoogle Scholar
Rattray, FP & Fox, PF 1999 Aspects of enzymology and biochemical properties of Brevibacterium linens relevant to cheese ripening: a review. Journal of Dairy Science 82 891909CrossRefGoogle ScholarPubMed
Reps, A 1993 Bacterial surface-ripened cheeses. In Cheese: Chemistry, Physics and Microbiology. Vol. 2: Major Cheese Groups. (Ed. Fox, PF) Chapman & Hall Publishers, London, UK., pp. 137172CrossRefGoogle Scholar