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Proteomic analysis of the effects of lutein on mammary gland metabolism in dairy cows

Published online by Cambridge University Press:  22 May 2018

Caihong Wang
Affiliation:
Institute of Dairy Science, Colleges of Animal Science, Zhejiang University, Hangzhou, 310058, P. R. China
Chong Wang
Affiliation:
College of Animal Science and technology, Zhejiang A & F University, Lin’ an 311300, P. R. China
Jianxin Liu
Affiliation:
Institute of Dairy Science, Colleges of Animal Science, Zhejiang University, Hangzhou, 310058, P. R. China
Hongyun Liu*
Affiliation:
Institute of Dairy Science, Colleges of Animal Science, Zhejiang University, Hangzhou, 310058, P. R. China
*
*For correspondence; e-mail: hyliu@zju.edu.cn

Abstract

The aim of the research reported in this Research Communication was to identify differentially expressed proteins in dairy cows with normal and lutein diet and to elucidate the mechanisms of lutein-induced effects on bovine mammary gland metabolism using a comparative proteomic approach. Thirty-three differentially expressed proteins were identified from mammary gland of control diet-fed and lutein diet-fed dairy cows. Among these proteins, 15 were upregulated and 18 were downregulated in the lutein group. Functional analysis of the differentially expressed proteins showed that increased blood flow, depressed glycolysis, enhanced lactose anabolism, decreased fatty acid oxidation and up-regulated beta lactoglobulin expression were connected with lutein addition. These results suggested that the increased blood flow, reduced glucose catabolism, enhanced capacity for milk lactose synthesis, depressed fatty acid catabolism and increased expression of antioxidantion related protein may be the prime factors contributing to the increased milk production and enhanced immune status in lutein-fed dairy cows. This study provides molecular mechanism of dietary lutein in regulating lactation of dairy cows.

Type
Research Article
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

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References

Bauman, DE & Griinari, JM 2003 Nutritional regulation of milk fat synthesis. Annual Review of Nutrition 23 203227CrossRefGoogle ScholarPubMed
Bernstein, PS, Khachik, F, Carvalho, LS, Muir, GJ, Zhao, DY & Katz, NB 2001 Identification and quantitation of carotenoids and their metabolites in the tissues of the human eye. Experimental Eye Research 72 215223CrossRefGoogle ScholarPubMed
Browner, MF, Taroni, F, Sztul, E & Rosenberg, LE 1989 Sequence analysis, biogenesis, and mitochondrial import of the a-subunit of rat liver propionyl-CoA carboxylase. Journal of Biological Chemistry 264 1268012685CrossRefGoogle Scholar
Carter, DC & Ho, JX 1994 Structure of serum albumin. Advances in Protein Chemistry 45 153203CrossRefGoogle ScholarPubMed
Chen, X, Kowal, P, Hamad, S, Fan, HN & Wang, PG 1999 Cloning, expression and characterization of a UDP-galactose 4-epimerase from Escherichia coli. Biotechnology Letters 21 11311135CrossRefGoogle Scholar
Levy, LW 2001 Trans-xanthophyll ester concentrates of enhanced purity and methods of making same. (Inventor: LW Levy). Inexa Industria Extractora. C. A. U.S. Patent No. 6,191,293Google Scholar
Liu, HC, Chen, WL, Mao, SJ 2007 Antioxidant nature of bovine milk beta-lactoglobulin. Journal of Dairy Science 90 547555CrossRefGoogle ScholarPubMed
Mangels, AR, Holden, JM, Beecher, GR, Forman, MR & Lanza, E 1993 Carotenoid content of fruits and vegetables: an evaluation of analytic data. American Dietetic Association 93 284296CrossRefGoogle ScholarPubMed
Moraes, ML, Ribeiro, AML, Santin, E & Klasing, KC 2016 Effects of conjugated linoleic acid and lutein on the growth performance and immune response of broiler chickens. Poultry Science 95 237246CrossRefGoogle ScholarPubMed
Wang, MX, Jiao, JH, Li, ZY, Liu, RR, Shi, Q & Ma, L 2013 Lutein supplementation reduces plasma lipid peroxidation and C-reactive protein in healthy nonsmokers. Atherosclerosis 227 380385CrossRefGoogle ScholarPubMed
Weed, RI, Reed, CF & Berg, G 1963 Is hemoglobin an essential structural component of human erythrocyte membranes? Journal of Clinical Investigation 42 581588CrossRefGoogle ScholarPubMed
Wu, YP, Zhou, JY, Zhang, X, Zheng, XJ, Jiang, XT, Shi, LX, Wei, Y & Wang, JH 2009 Optimized sample preparation for two-dimensional gel electrophoresis of soluble proteins from chicken bursa of fabricius. Proteome Science 7 69746977CrossRefGoogle ScholarPubMed
Xu, CZ, Wang, HF, Yang, JY, Wang, JH, Duan, ZY, Wang, C, Liu, JX & Lao, Y 2014 Effects of feeding lutein on production performance, antioxidative status and milk quality of high-yielding dairy cows. Journal of Dairy Science 97 71447150CrossRefGoogle ScholarPubMed
Zommara, M, Toubo, H, Sakono, M& Imaizumi, K 1998. Prevention of peroxidative stress in rats fed on a low vitamin E-containing diet by supplementing with a fermented bovine milk whey preparation: effect of lactic acid and beta-lactoglobulin on the antiperoxidative action. Bioscience Biotechnology and Biochemistry 62 710717CrossRefGoogle ScholarPubMed