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Liver X receptor α participates in LPS-induced reduction of triglyceride synthesis in bovine mammary epithelial cells

Published online by Cambridge University Press:  02 December 2020

Jianfa Wang ,
Shuai Lian ,
Jun Song ,
Hai Wang ,
Xu Zhang ,
Xianjing He ,
Dandan Hao  and
Rui Wu
Jianfa Wang*
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 2 Xinyang Road, Sartu District, Daqing163319, P.R. China Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, No. 2 Xinyang Road, Sartu District, Daqing163319, P.R. China
Shuai Lian
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 2 Xinyang Road, Sartu District, Daqing163319, P.R. China Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, No. 2 Xinyang Road, Sartu District, Daqing163319, P.R. China
Jun Song
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 2 Xinyang Road, Sartu District, Daqing163319, P.R. China Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, No. 2 Xinyang Road, Sartu District, Daqing163319, P.R. China
Hai Wang*
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 2 Xinyang Road, Sartu District, Daqing163319, P.R. China Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, No. 2 Xinyang Road, Sartu District, Daqing163319, P.R. China
Xu Zhang
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 2 Xinyang Road, Sartu District, Daqing163319, P.R. China Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, No. 2 Xinyang Road, Sartu District, Daqing163319, P.R. China
Xianjing He
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 2 Xinyang Road, Sartu District, Daqing163319, P.R. China
Dandan Hao
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 2 Xinyang Road, Sartu District, Daqing163319, P.R. China
Rui Wu
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 2 Xinyang Road, Sartu District, Daqing163319, P.R. China Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, No. 2 Xinyang Road, Sartu District, Daqing163319, P.R. China
*
Author for correspondence: Jianfa Wang, Email: wjflw@sina.com
Author for correspondence: Jianfa Wang, Email: wjflw@sina.com
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Abstract

Lipopolysaccharides (LPS) could induce milk fat depression via regulating the body and blood fat metabolism. However, it is not completely clear how LPS might regulate triglyceride synthesis in dairy cow mammary epithelial cells (DCMECs). DCMECs were isolated and purified from dairy cow mammary tissue and treated with LPS. The level of triglyceride synthesis, the expression and activity of the liver X receptor α (LXRα), enzymes related to de novo fatty acid synthesis, and the expression of the fatty acid transporters were investigated. We found that LPS decreased the level of triglyceride synthesis via a down-regulation of the transcription, translation, and nuclear translocation level of the LXRα. The results also indicated that the transcription level of the LXRα target genes, sterol regulatory element binding protein 1 (SREBP1), fatty acid synthetase (FAS), acetyl-CoA carboxylase-1 (ACC1), were significantly down-regulated in DCMECs after LPS treatment. Our data may provide new insight into the mechanisms of milk fat depression caused by LPS.

Keywords

bovine mammary epithelial cellslipopolysaccharidesliver X receptor αtriglyceride
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 4 , November 2020 , pp. 456 - 462
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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