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In vitro antimicrobial and antibiofilm activity of phage cocktail against Mammaliicoccus sciuri, a causative agent of bovine mastitis

Published online by Cambridge University Press:  27 August 2024

Puthiya Maliyekkal Shahana Shirin ,
Archana Chandran Open the ORCID record for Archana Chandran [Opens in a new window] ,
Pathiyarathvalappill Subrahmanian Surabhi ,
Ramachandran Latha Rathish  and
Mundakka Paramban Rahila
Puthiya Maliyekkal Shahana Shirin
Affiliation:
Department of Biotechnology, Cochin University of Science and technology, South Kalamassery, Kochi, Kerala, India
Archana Chandran*
Affiliation:
Department of Dairy Microbiology, College of Dairy Science and Technology, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala, India
Pathiyarathvalappill Subrahmanian Surabhi
Affiliation:
PG Department of Biology, Sree Narayana Guru College, Chellanur, Calicut University, Malappuram, Kerala, India
Ramachandran Latha Rathish
Affiliation:
Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala, India
Mundakka Paramban Rahila
Affiliation:
Department of Dairy Chemistry, College of Dairy Science and Technology, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala, India
*
Corresponding author: Archana Chandran; Email: archanac@kvasu.ac.in
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Abstract

In this research paper the in vitro antimicrobial and antibiofilm activity of phage cocktail against the coagulase negative Mammaliicoccus sciuri was investigated. Three M. sciuri isolates obtained from clinical bovine mastitis samples were characterized and identified by 16S rRNA gene sequencing. Bacteriophages with lytic activity against M. sciuri isolates were isolated from dairy farm effluents. Two typical phages were isolated using standard enrichment and plaque assay techniques, purified by polyethylene glycol precipitation, and morphologically characterized based on shape and size using transmission electron microscopy. This was followed by determination of host range using spot tests and stability to varying temperature, pH and UV treatment. The phage cocktail suppressed bacterial activity within 30 min of exposure. Crystal violet assay showed that the tested phages and their cocktail significantly reduced the biofilm biomass of all three M. sciuri strains compared to the untreated control in vitro within 24 h with a single dosing. Transmission electron micrography of the purified phage particle revealed an icosahedral head and a rigid contractile tail, characteristic of the class Caudoviricetes. The findings open new avenues in phage-based antimicrobial approaches for controlling contagious and teat skin opportunistic bacteria causing bovine mastitis.

Keywords

Mammaliicoccus sciuriBovine mastitisAntibiotic resistanceBacteriophagePhage therapyAntibiofilmBiocontrol
Type
Research Article
Information
Journal of Dairy Research , First View , pp. 1 - 6
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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