Hostname: page-component-8448b6f56d-jr42d Total loading time: 0 Render date: 2024-04-23T05:10:13.542Z Has data issue: false hasContentIssue false

Identification of alleles and genotypes of beta-casein with DNA sequencing analysis in Chinese Holstein cow

Published online by Cambridge University Press:  07 September 2016

Ronghua Dai
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Yu Fang
Affiliation:
Shanghai Personal Biotechnology Co., Ltd., Shanghai 200240, P. R. China
Wenjing Zhao
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Shuyun Liu
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Jinmei Ding
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Ke Xu
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Lingyu Yang
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Chuan He
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
Fangmei Ding
Affiliation:
Shanghai Personal Biotechnology Co., Ltd., Shanghai 200240, P. R. China
He Meng*
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China
*
*For correspondence; e-mail: menghe@sjtu.edu.cn

Abstract

The study reported in this Regional Research Communication aimed to analyse the genetic polymorphisms of β-casein in Chinese Holstein cows. β-casein has received considerable research interest in the dairy industry and animal breeding in recent years as a source not only of high quality protein, but also of bioactive peptides that may be linked to health effects. Morever, the polymorphic nature of β-casein and its association with milk production traits, composition, and quality also attracted several efforts in evaluating the allelic distribution of β-casein locus as a potential dairy trait marker. However, few data on beta-casein variants are available for the Chinese Holstein cow. In the present paper, one hundred and thirty three Holstein cows were included in the analysis. Results revealed the presence of 5 variants (A1, A2, A3, B and I), preponderance of the genotype A1A2 (0·353) and superiorities of A1/A2 alleles (0·432 and 0·459, respectively) in the population. Sequence analysis of β-casein gene in the cows showed four nucleotide changes in exon 7. Our study can provide reference and guidance for selection for superior milk for industrial applications and crossbreeding and genetic improvement programmes.

Type
Research Article
Copyright
Copyright © Proprietors of Journal of Dairy Research 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Barroso, A, Dunner, S & Cañón, J 1999 Technical note: use of PCR-single-strand conformation polymorphism analysis for detection of bovine beta-casein variants A1, A2, A3, and B. Journal of Animal Science 77 26292632 Google Scholar
Bech, AM & Kristiansen, KR 1990 Milk protein polymorphism in Danish dairy cattle and the influence of genetic variants on milk yield. Journal of Dairy Research 57 5362 Google Scholar
Bell, SJ, Grochoski, GT & Clarke, AJ 2006 Health Implications of milk containing β-Casein with the A2 genetic variant. Critical Reviews in Food Science and Nutrition 46 93100 Google Scholar
Boettcher, PJ, Caroli, A, Stella, A, Chessa, A, Budelli, E, Canavesi, F, Ghiroldi, S & Pagnacco, G 2004 Effects of casein haplotypes on milk production traits in Italian Holstein and Brown Swiss cattle. Journal of Dairy Science 87 43114317 CrossRefGoogle ScholarPubMed
Cieślińska, A, Kostyra, E, Kostyra, H, Oleński, K, Fiedorowicz, E & Kamiński, S 2012 Milk from cows of different β-casein genotypes as a source of β-casomorphin-7. International Journal of Food Sciences and Nutrition 63 426430 Google Scholar
Clemens, RA 2011 Milk A1 and A2 peptides and diabetes. Nestle Nutrition Institute Workshop Series: Pediatric Program 67 187195 CrossRefGoogle ScholarPubMed
Ehrmann, BS, Bartenschlager, H & Geldermann, H 1997 Quantification of gene effects on single milk proteins in selected groups of dairy cows. Journal of Animal Breeding and Genetics 114 121132 Google Scholar
Elliott, RB, Harris, DP, Hill, JP, Bibby, NJ & Wasmuth, HE 1999 Type I (insulin-dependent) diabetes mellitus and cow milk: casein variant consumption. Diabetologia 42 292296 Google Scholar
Farrell, HMJ, Jimenez-Flores, R, Bleck, GT, Brown, EM, Butler, JE, Creamer, LK, Hicks, CL, Hollar, CM, Ng-Kwai-Hang, KF & Swaisgood, HE 2004 Nomenclature of the proteins of cows’ milk–sixth revision. Journal of Dairy Science 87 16411674 Google Scholar
Freyer, BG, Liu, Z, Erhardt, G & Panicke, L 1999 Casein polymorphism and relation between milk production traits. Journal of Animal Breeding and Genetics 166 8797 Google Scholar
Gallinat, JL, Qanbari, S, Drögemüller, C, Pimentel, ECG, Thaller, G & Tetens, J 2013 DNA-based identification of novel bovine casein gene variants. Journal of Dairy Science 96 699709 Google Scholar
Givens, I, Aikman, P, Gibson, T & Brown, R 2013 Proportions of A1, A2, B and C β-casein protein variants in retail milk in the UK. Food Chemistry 139 549552 CrossRefGoogle Scholar
Haq, U, Kapila, R, Shandilya, UK & Kapila, S 2014 Impact of milk derived β-casomorphins on physiological functions and trends in research: a review. International Journal of Food Properties 17 17261741 CrossRefGoogle Scholar
Kamiński, S, Cieoelińska, A & Kostyra, E 2007 Polymorphism of bovine beta-casein and its potential effect on human health. Journal of Applied Genetics 48 189198 Google Scholar
Lipkin, E, Tal-Stein, R, Friedmann, A & Soller, M 2008 Effect of quantitative trait loci for milk protein percentage on milk protein yield and milk yield in Israeli Holstein dairy cattle. Journal of Dairy Science 91 16141627 CrossRefGoogle ScholarPubMed
Manga, I & Dvořák, J 2010 TaqMan allelic discrimination assay for A1 and A2 alleles of the bovine CSN2 gene. Czech Journal of Animal Science 55 307312 Google Scholar
McLachlan, CNS 2001 β-casein A1, ischaemic heart disease mortality, and other illnesses. Medical Hypotheses 56 262272 CrossRefGoogle ScholarPubMed
Nguyena, DD, Johnsona, SK, Busettib, F & Solaha, VA 2015 Formation and degradation of beta-casomorphins in dairy processing. Critical Reviews in Food Science and Nutrition 55 19551967 Google Scholar
Oleński, K, Kamiński, S, Szyda, J & Cieslinska, A 2010 Polymorphism of the beta-casein gene and its associations with breeding value for production traits of Holstein–Friesian bulls. Livestock Science 131 137140 Google Scholar
Oleński, K, Cieślińska, A, Suchocki, T, Szyda, J & Kamiński, S 2012 Polymorphism in coding and regulatory sequences of beta-casein gene is associated with milk production traits in Holstein-Friesian cattle. Animal Science Papers and Reports 30 512 Google Scholar
Singh, LV, Jayakumar, S, Sharma, A, Gupta, SK, Dixit, SP, Gupta, N & Gupta, SC 2015 Comparative screening of single nucleotide polymorphisms in β-casein and κ-casein gene in different livestock breeds of India. Meta Gene 4 8591 CrossRefGoogle ScholarPubMed
Sodhi, M, Mukesh, M, Mishra, BP, Kishore, A, Prakash, B, Kapil, R, Khate, K, Kataria, RS & Joshi, BK 2012 Screening of taurine and crossbred breeding bulls for A1/A2 variants of β-casein gene. Indian Journal of Animal Sciences 82 183186 Google Scholar
Sun, DX, Fan, XH, Xie, Y, Chu, Q, Sun, Y, Zhang, Y, Zhang, SL, Gong, WJ, Chen, SH, Li, YH, Shi, WH & Zhang, Y 2011 Distribution of recessive genetic defect carriers in Chinese Holstein. Journal of Dairy Science 94 56955698 CrossRefGoogle ScholarPubMed
Swaissgood, HE 1992 Chemistry of the caseins. In Advanced Dairy Chemistry-1: Proteins, pp. 6377 (Ed. Fox, PF). London: Elsevier Google Scholar
Vallas, M, Kaart, T, Varv, S, Parna, K, Joudu, I, Viinalass, H & Parna, E 2012 Composite β-κ-casein genotypes and their effect on composition and coagulation of milk from Estonian Holstein cows. Journal of Dairy Science 95 67606769 Google Scholar
Visker, MHPW, Dibbits, BW, Kinders, SM, Van Valenberg, HJF, Van Arendonk, JAM & Bovenhuis, H 2011 Association of bovine b-casein protein variant I with milk production and milk protein composition. Animal Genetics 42 212218 Google Scholar
Supplementary material: PDF

Dai supplementary material

Table S1

Download Dai supplementary material(PDF)
PDF 151.3 KB