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Activity against Listeria monocytogenes of human milk during lactation. A preliminary study

Published online by Cambridge University Press:  29 January 2008

Iván López-Expósito ,
María Asunción Manso ,
Rosina López-Fandiño  and
Isidra Recio
Iván López-Expósito
Affiliation:
Instituto de Fermentaciones Industriales (CSIC). C/Juan de la Cierva, 3, 28006 Madrid, Spain
María Asunción Manso
Affiliation:
Instituto de Fermentaciones Industriales (CSIC). C/Juan de la Cierva, 3, 28006 Madrid, Spain
Rosina López-Fandiño
Affiliation:
Instituto de Fermentaciones Industriales (CSIC). C/Juan de la Cierva, 3, 28006 Madrid, Spain
Isidra Recio*
Affiliation:
Instituto de Fermentaciones Industriales (CSIC). C/Juan de la Cierva, 3, 28006 Madrid, Spain
*
*For correspondence; e-mail: recio@ifi.csic.es
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Abstract

Human milk samples from three healthy donors were investigated in order to evaluate the antibacterial activity during lactation against Escherichia coli ATCC 25922 and Listeria monocytogenes. The concentration of the main human-milk antimicrobial proteins (lactoferrin (LF), lysozyme (LZ) and secretory immunoglobulin A (sIgA)) was determined by ELISA. Results showed that human milk exhibited antibacterial activity against List. monocytogenes, although it was weakly active against Esch. coli ATCC 25922. The observed antilisterial activity was positively correlated with LZ concentration. In addition, the effect of gastrointestinal proteases, at different pH conditions, that prevail in the stomach of infants (pH 2·0–6·5), on antilisterial activity and protein degradation was evaluated. Hydrolysis with pepsin at pH 4·0–6·5, followed by treatment with pancreatic enzymes, resulted in a decreased hydrolysis of LZ, LF and sIgA and an enhanced antibacterial activity against List. monocytogenes. It is suggested that partial degradation of certain milk proteins at the gastrointestinal level may produce peptides that could act synergistically with the remnant intact proteins.

Keywords

Antibacterial activityhuman milkListeria monocytogenesgastrointestinal proteases
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 1 , February 2008 , pp. 24 - 29
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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References

Andersson, Y, Lindquist, S, Lagerqvist, C & Hernell, O 2000 Lactoferrin is responsible for the fungistatic effect of human milk. Early Human Development 59 95–105CrossRefGoogle ScholarPubMed
Afify, AM, Mohamed, MA, Abdel-Salam, AM & Abd El-Azim, SA 2003 Electrophoretic analysis of colostrums and mature Egyptian human milk using SDS-polyacrylamide gel. Milchwissenschaft 58 583–585Google Scholar
Bellamy, W, Takase, M, Wakabayashi, H, Kawase, K & Tomita, M 1992 Antibacterial spectrum of lactoferricin B, a potent bactericidal peptide derived from the N-terminal region of bovine lactoferrin. Journal of Applied Bacteriology 73 472–479CrossRefGoogle ScholarPubMed
Chatterton, DEW, Rasmussen, JT, Heegaard, CW, Sorensen, ES & Petersen, TE 2004 In vitro digestion of novel milk protein ingredients for use in infant formulas: Research on biological functions. Trends in Food Science and Technology 15 373–383CrossRefGoogle Scholar
Davidson, LA & Lönnerdal, B 1987 Persistence of human milk proteins in the breast-fed infants. Acta Paediatrica Scandinavica 76 733–740CrossRefGoogle Scholar
Dolan, SA, Boesman-Fikelstein, M & Finkelstein, A 1986 Antimicrobial activity of human milk against pediatric pathogens. Journal of Infection Disease 154 722–725CrossRefGoogle ScholarPubMed
Dolan, SA, Boesman-Fikelstein, M & Finkelstein, A 1989 Inhibition of enteropathogenic bacteria by human milk whey in vitro. The Pediatric Infectious Disease Journal 8 430–436CrossRefGoogle ScholarPubMed
Emmett, PM & Rogers, IS 1997 Properties of human milk and their relationship with maternal nutrition. Early Human Development 49 S7–S28CrossRefGoogle ScholarPubMed
Ellison, RT & Giehl, TJ 1991 Killing of Gram-negative bacteria by lactoferrin and lysozyme. Journal of Clinical Investigation 88 1080–1091CrossRefGoogle ScholarPubMed
Ferranti, P, Traisci, MV, Picariello, G, Nasi, A, Boschi, V, Siervo, M, Falconi, C, Chianese, L & Addeo, F 2004 Casein proteolysis in human milk: Tracing the pattern of casein breakdown and the formation of potential bioactive peptides. Journal of Dairy Research 71 74–87CrossRefGoogle ScholarPubMed
Floris, R, Recio, I, Berkhout, B & Visser, S 2003 Antibacterial and antiviral effects of milk proteins and derivatives thereof. Current Pharmaceutical Design 9 1257–1275CrossRefGoogle Scholar
Goldman, AS, Ham Pong, AJ & Goldblum, RM 1985 Host defenses: development and maternal contributions. Advanced Pediatric 32 71–100CrossRefGoogle ScholarPubMed
Hamosh, M 1998 Protective function of proteins and lipids in human milk. Biology of the Neonate 74 163–176CrossRefGoogle ScholarPubMed
Hanson, LA, Korotkova, M, Haversen, L, Mattsby-Baltzer, I, Hahn-Zoric, M, Silfverdal, SA, Strandvik, B & Telemo, E 2002 Breast-feeding, a complex support system for the offspring. Pediatrics International 44 347–352Google ScholarPubMed
Hennart, PH, Brasseur, DJ, Delogne-Desnoeck, JB, Dramaix, MM & Robyn, CE 1991 Lysozyme, lactoferrin and secretory immunoglobulin A content in breast milk: influence of duration of lactation, nutrition status, prolactin status and parity of mother. American Journal of Clinical Nutrition 53 32–39CrossRefGoogle ScholarPubMed
Hernández-Ledesma, B, Quirós, A, Amigo, L & Recio, I 2007 Identification of bioactive peptides after digestion of human milk and infant formula with pepsin and pancreatin. International Dairy Journal 17 42–49CrossRefGoogle Scholar
Isaacs, CE 2005 Human milk inactivates pathogens individually, additively and synergistically. Journal of Nutrition 135 1286–1288CrossRefGoogle ScholarPubMed
Kuwata, H, Yip, TT, Tomita, M & Hutchens, TW 1998 Direct evidence of the generation in human stomach of an antimicrobial peptide domain (lactoferricin) from ingested lactoferrin. Biochimica et Biophysica Acta 1429 129–141CrossRefGoogle ScholarPubMed
Liepke, C, Zucht, HD, Forssmann, WG & Ständker, L 2001 Purification of novel peptide antibiotics from human milk. Journal of Chromatography A 752 369–377CrossRefGoogle ScholarPubMed
Lönnerdal, B & Iyer, S 1995 Lactoferrin: molecular structure and biological function. Annual Review of Nutrition 15 93–110CrossRefGoogle ScholarPubMed
Lönnerdal, B 2003 Nutritional and physiologic significance of human milk proteins. American Journal of Clinical Nutrition 77 1537S–1543SCrossRefGoogle ScholarPubMed
López-Expósito, I, Gómez-Ruiz, JA, Amigo, L & Recio, I 2006 Identification of antibacterial peptides from ovine αs2-casein. International Dairy Journal 16 1072–1080CrossRefGoogle Scholar
López-Expósito, I, Pellegrini, A, Amigo, L & Recio, I 2008 Synergistic effect between different milk-derived peptides and proteins. Journal of Dairy Science (In press)CrossRefGoogle ScholarPubMed
Mason, S 1962 Some aspects of gastric function in the newborn. Archives of Disease in Childhood 37 87–91CrossRefGoogle ScholarPubMed
Mitchell, DJ, McClure, BG & Tubman, TRJ 2001 Simultaneous monitoring of gastric pH and oesophageal pH reveals limitations of conventional oesophageal pH monitoring in milk fed infants. Archives of Disease in Childhood 84 273–276CrossRefGoogle ScholarPubMed
Montagne, PM, Trégoat, VS, Cuillière, ML, Béné, MC & Faure, GC 2000 Measurement of nine human milk proteins by nephelometric immunoassays: application to the determination of mature milk protein profile. Clinical Biochemistry 33 181–186CrossRefGoogle Scholar
Newburg, DS 2005 Innate immunity and human milk. Journal of Nutrition 135 1308–1312CrossRefGoogle ScholarPubMed
Newman, J 1995 How breast milk protects newborns. Scientific American 273 76–79CrossRefGoogle ScholarPubMed
Pakkanen, R & Aalto, J 1997 Growth factors and antimicrobial factors of bovine colostrums. International Dairy Journal 7 285–297CrossRefGoogle Scholar
Redhead, K, Hill, T & Mulloy, B 1990 Antimicrobial effect of human milk on Bordetella pertusis. FEMS Microbiology Letters 70 269–274Google Scholar
Solórzano-Santos, F, Castellanos-Cruz, C, Echaniz-Avilés, G & Arredondo-García, JL 1993 Antimicrobial activity of human calostrum against enteropathogens. A preliminary study. Revista Latinoamericana de Microbiología 35 1–6Google Scholar
Troost, FJ, Stejins, J, Saris, WHM & Brummer, RJM 2001 Gastric digestion of bovine lactoferrin in vivo in adults. Journal of Nutrition 131 2101–2104CrossRefGoogle ScholarPubMed
Vazquez-Boland, JA, Kuhn, M, Merche, P, Chakraborty, T, Dominguez-Bernal, G, Goebel, W, González-Zorn, B, Wehland, J & Kreft, J 2001 Listeria pathogenesis and molecular virulence determinants. Clinical Microbiology. Reviews 14 584–640CrossRefGoogle ScholarPubMed
Walker, WA 2004 The dynamic effects of breastfeeding on intestinal development and host defense. Advances in Experimental Medicine and Biology 554 155–170CrossRefGoogle ScholarPubMed
Ward, PP & Conneely, OM 2004 Lactoferrin: Role in iron homeostasis and host defense against microbial infection. Biometals 17 203–208CrossRefGoogle ScholarPubMed
Weaver, LT, Arthur, HML, Bunn, EG & Thomas, JE 1998 Human milk sIgA concentrations during the first year of lactation. Archives of Disease in Childhood 78 235–239CrossRefGoogle ScholarPubMed