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Isolation and identification of lactic acid bacteria in fresh plants and in silage from Opuntia and their effects on the fermentation and aerobic stability of silage

Published online by Cambridge University Press:  25 March 2020

G. A. Pereira ,
E. M. Santos ,
G. G. L. Araújo ,
J. S. Oliveira ,
R. M. A. Pinho Open the ORCID record for R. M. A. Pinho [Opens in a new window] ,
A. de M. Zanine Open the ORCID record for A. de M. Zanine [Opens in a new window] ,
A. F. N. Souza ,
A. J. S. Macedo ,
J. M. C. Neto  and
T. V. C. Nascimento Open the ORCID record for T. V. C. Nascimento [Opens in a new window]
G. A. Pereira
Affiliation:
Department of Animal Science, Federal University of Paraíba, Areia, Paraíba, Brazil
E. M. Santos
Affiliation:
Department of Animal Science, Federal University of Paraíba, Areia, Paraíba, Brazil
G. G. L. Araújo
Affiliation:
Brazilian Agricultural Research Corporation, EMBRAPA Semiarid, Petrolina, Pernambuco, Brazil
J. S. Oliveira
Affiliation:
Department of Animal Science, Federal University of Paraíba, Areia, Paraíba, Brazil
R. M. A. Pinho
Affiliation:
Department of Animal Science, Federal University of Maranhão, Chapadinha, Maranhão, Brazil
A. de M. Zanine*
Affiliation:
Department of Animal Science, Federal University of Maranhão, Chapadinha, Maranhão, Brazil
A. F. N. Souza
Affiliation:
Department of Animal Science, Federal University of Paraíba, Areia, Paraíba, Brazil
A. J. S. Macedo
Affiliation:
Department of Animal Science, Federal University of Paraíba, Areia, Paraíba, Brazil
J. M. C. Neto
Affiliation:
Department of Animal Science, Federal University of Paraíba, Areia, Paraíba, Brazil
T. V. C. Nascimento
Affiliation:
Department of Animal Science, Federal University of Maranhão, Chapadinha, Maranhão, Brazil
*
Author for correspondence: A. de M. Zanine, E-mail: anderson.zanine@ibest.com.br
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Abstract

The current study aimed to select the strains of lactic acid bacteria (LAB) isolated from forage cactus plants and silage and assess their effects on silage fermentation and aerobic stability. Forty wild isolates from plant and cactus silage, classified as LAB, were evaluated for metabolite production and identified by 16S ribosomal DNA sequencing. These wild isolates were identified as Lactobacillus plantarum, Weissella cibaria, Weissella confusa and Weissella paramesenteroides and the LAB populations differed among the silage. The use of microbial inoculants did not influence gas or effluent losses in forage cactus silage. The silage inoculated with the microbial strain GP15 showed the highest number of LAB populations. The amounts of water-soluble carbohydrates (WSC) and ammonia nitrogen differed among the silage. The silage inoculated with the GP1 strain presented the highest WSC. Populations of enterobacteria and yeasts and moulds were below the minimum detection limit (<2.0 log cfu/g silage) in all the silage studied. The predominant action of inoculants was to maximize dry matter recovery of the silage, which could be the criterion adopted to select the strains of LAB for use as inoculants in Opuntia silage.

Keywords

Lactobacillus plantarumribosomal DNA sequenceWeissella cibariaWeissella confusaWeissella paramesenteroides
Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 157 , Issue 9-10 , December 2019 , pp. 684 - 692
Copyright
Copyright © Cambridge University Press 2020

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