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In vitro maturation and attempted fertilization of cattle follicular oocytes

Published online by Cambridge University Press:  27 March 2009

J. Sreenan
J. Sreenan
Affiliation:
Faculty of Agriculture, University College Dublin
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Summary

Maturation studies were carried out on 241 cattle oocytes. Media used were bovine follicular fluid and growth medium. Both media supported a resumption of meiosis in ca. 80 % of cases, but growth medium proved superior in terms of final maturation.

Fertilization attempts were carried out on 564 oocytes following culture (27–32 h) in growth medium. In vitro fertilization attempts (107 oocytes) and in vivo fertilization attempts (82 oocytes) in the rabbit yielded negative results. Following in vivo fertilization attempts in the ewe, ova containing pronuclei as well as apparently normal cleaved ova were recovered.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 75 , Issue 3 , December 1970 , pp. 393 - 396
Copyright
Copyright © Cambridge University Press 1970

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