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Identification and characterization of two faba bean (Vicia faba L.) WRKY transcription factors and their expression analysis during salt and drought stress

Published online by Cambridge University Press:  03 November 2016

G. ABID ,
Y. MUHOVSKI ,
D. MINGEOT ,
M. N. SAIDI ,
M. AOUIDA ,
I. AROUA ,
M. M'HAMDI ,
F. BARHOUMI ,
S. REZGUI  and
M. JEBARA
G. ABID*
Affiliation:
Laboratory of Legumes, University of Tunis El Manar, Center of Biotechnology of Borj Cedria, 901, B-2050 Hammam-Lif, Tunisia
Y. MUHOVSKI
Affiliation:
Department of Life Sciences, Unit of Biological Engineering, Walloon Agricultural Research Centre, Chaussée de Charleroi, 234, B-5030 Gembloux, Belgium
D. MINGEOT
Affiliation:
Department of Life Sciences, Unit of Biological Engineering, Walloon Agricultural Research Centre, Chaussée de Charleroi, 234, B-5030 Gembloux, Belgium
M. N. SAIDI
Affiliation:
Center of Biotechnology of Sfax, Route Sidi Mansour Km 4, B.P 1177, 3018 Sfax, Tunisia
M. AOUIDA
Affiliation:
Laboratory of Legumes, University of Tunis El Manar, Center of Biotechnology of Borj Cedria, 901, B-2050 Hammam-Lif, Tunisia
I. AROUA
Affiliation:
Laboratory of Legumes, University of Tunis El Manar, Center of Biotechnology of Borj Cedria, 901, B-2050 Hammam-Lif, Tunisia
M. M'HAMDI
Affiliation:
Higher Agronomic Institute of Chott Mariem, BP 47, 4042 Chott Mariem, Sousse, Tunisia
F. BARHOUMI
Affiliation:
Laboratory of Legumes, University of Tunis El Manar, Center of Biotechnology of Borj Cedria, 901, B-2050 Hammam-Lif, Tunisia
S. REZGUI
Affiliation:
Laboratory of Legumes, University of Tunis El Manar, Center of Biotechnology of Borj Cedria, 901, B-2050 Hammam-Lif, Tunisia
M. JEBARA
Affiliation:
Laboratory of Legumes, University of Tunis El Manar, Center of Biotechnology of Borj Cedria, 901, B-2050 Hammam-Lif, Tunisia
*
*To whom all correspondence should be addressed. Email: gha_abid@yahoo.fr
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Summary

Drought and salinity are two major environmental factors limiting faba bean growth, leading to considerable reduction in their productivity. The WRKY gene family act as major transcription factors that might play an important role in abiotic stress tolerance. In the present study, two partial sequences sharing significant homology with known WRKY genes were isolated from faba bean by polymerase chain reaction (PCR) amplification using degenerate primers targeting the well-conserved WRKY domain. The isolated WRKY gene fragments were designated as VfWRKY1 and VfWRKY2 showing 62% similarity between them. Sequence and phylogenetic analyses revealed that VfWRKY1 and VfWRKY2 belong to WRKY group I and could be grouped with their orthologues from other plant species. The gene expression profile of VfWRKY1 and VfWRKY2 in faba bean showed that they are significantly accumulated in various plant organs. Further, quantitative real-time PCR analysis showed that both transcripts were responsive to drought and salt stress, and also they are genotype dependent, meaning that different faba bean cultivars respond in a different way to drought and salt challenge. The expression patterns obtained suggest the important roles of VfWRKY1 and VfWRKY2 in drought and salt stress response and tolerance. This knowledge might be helpful in the identification of drought-tolerant cultivars and provide potential candidate markers for faba bean breeding in order to develop osmotic-stress-tolerant cultivars.

Type
Crops and Soils Research Papers
Information
The Journal of Agricultural Science , Volume 155 , Issue 5 , July 2017 , pp. 791 - 803
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Copyright
Copyright © Cambridge University Press 2016 

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